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arXiv 1705.00295cond-mat.mes-hall
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石墨烯晶体管在神经元网络中的离子通道传感

Sensing ion channels in neuronal networks with graphene transistors

Farida Veliev, Dipankar Kalita, Antoine Bourrier, Tiphaine Belloir, Anne Briançon-Marjollet, Mireille Albrieux, Vincent Bouchiat, Cécile Delacour

摘要:

石墨烯是一种原子级薄的蜂窝状碳晶格,是一种高度导电的2D材料,其暴露的电子结构为传感提供了理想的平台。它的生物相容性,灵活性和化学惰性的性质与悬空键的缺乏,提供了新的视角与生物元素直接接口。结合其卓越的电子和光学特性,石墨烯成为一种非常有前途的生物电子材料。在石墨烯的成功生物整合中,通过人工膜通道和产电细胞中的细胞外动作电位检测离子电流为神经元活动的高空间分辨率和宽场成像铺平了道路。然而,各种问题,包括低信号幅度,限制和随机性的神经元信号相关联的复杂的结构和神经网络的互连性仍然需要克服。最近,在CVD石墨烯中发现的晶界被证明可以大大增加石墨烯晶体管的灵敏度,从而提供纳米级的传感位置。在这里,我们证明了液体门控石墨烯场效应晶体管(G-FET)的能力,海马神经元生长的单离子通道活性的实时检测。依赖于药物和参考电位门控,并发现兼容的纳米级耦合的几个离子通道石墨烯晶界。

英文摘要:

Graphene, the atomically-thin honeycomb carbon lattice, is a highly conducting 2D material whose exposed electronic structure offers an ideal platform for sensing. Its biocompatible, flexible, and chemically inert nature associated to the lack of dangling bonds, offers novel perspectives for direct interfacing with bioelements. When combined with its exceptional electronic and optical properties, graphene becomes a very promising material for bioelectronics. Among the successful bio-integrations of graphene, the detection of ionic currents through artificial membrane channels and extracellular action potentials in electrogenic cells have paved the road for the high spatial resolution and wide-field imaging of neuronal activity. However, various issues including the low signals amplitude, confinement and stochasticity of neuronal signals associated to the complex architecture and interconnectivity of neural networks should be still overcome. Recently, grain boundaries found in CVD graphene were shown to drastically increase the sensitivity of graphene transistors providing nanoscale sensing sites. Here we demonstrate the ability of liquid-gated graphene field effect transistors (G-FET) on which hippocampal neurons are grown for real-time detection of single ion channels activity. Dependence upon drugs and reference potential gating is presented and is found compatible with the nanoscale coupling of a few ion channels to graphene grain boundaries.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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