使用具有闭式解的角向叠层衍射成像的高效、千兆像素级、无像差的全玻片扫描仪
Efficient, gigapixel-scale, aberration-free whole slide scanner using angular ptychographic imaging with closed-form solution
AI总结：
本文提出一种基于闭式解角向叠层衍射成像的全玻片成像系统WSI-APIC，通过GPU加速算法实现数字像差校正与分辨率提升，并利用倾斜非相干照明跳过背景扫描，最终以10x物镜实现772nm分辨率和80千兆像素无像差成像。
摘要：
全玻片成像在活检或手术样本的横截面上提供了宽广的视场（FOV），显著促进了病理分析和临床诊断。这些能够详细可视化细胞和组织结构的高质量图像，对于有效的患者护理和治疗规划至关重要。为了在病理学应用中获得此类高质量图像，需要具有高空间带宽积、无像差且无需z轴扫描的扫描仪。在此，我们报告了一种基于具有闭式解的角向叠层衍射成像（WSI-APIC）的全玻片成像系统，它提供了高效、数十千兆像素、大视场、无像差的成像。WSI-APIC利用倾斜非相干照明进行初始高级分割，从而绕过对背景区域的不必要扫描，提高了图像采集效率。GPU加速的APIC算法通过有效的数字像差校正和提升的光学分辨率，解析重建出相位图像。此外，基于尺度不变特征变换的自动拼接技术确保了全玻片相位图像的无缝拼接。在我们的实验中，WSI-APIC使用10x/0.25 NA物镜实现了772 nm的光学分辨率，并为标准的76.2 mm x 25.4 mm显微玻片捕获了80千兆像素的无像差相位图像。
英文摘要：
Whole slide imaging provides a wide field-of-view (FOV) across cross-sections of biopsy or surgery samples, significantly facilitating pathological analysis and clinical diagnosis. Such high-quality images that enable detailed visualization of cellular and tissue structures are essential for effective patient care and treatment planning. To obtain such high-quality images for pathology applications, there is a need for scanners with high spatial bandwidth products, free from aberrations, and without the requirement for z-scanning. Here we report a whole slide imaging system based on angular ptychographic imaging with a closed-form solution (WSI-APIC), which offers efficient, tens-of-gigapixels, large-FOV, aberration-free imaging. WSI-APIC utilizes oblique incoherent illumination for initial high-level segmentation, thereby bypassing unnecessary scanning of the background regions and enhancing image acquisition efficiency. A GPU-accelerated APIC algorithm analytically reconstructs phase images with effective digital aberration corrections and improved optical resolutions. Moreover, an auto-stitching technique based on scale-invariant feature transform ensures the seamless concatenation of whole slide phase images. In our experiment, WSI-APIC achieved an optical resolution of 772 nm using a 10x/0.25 NA objective lens and captures 80-gigapixel aberration-free phase images for a standard 76.2 mm x 25.4 mm microscopic slide.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
