具备鲁棒像差校正的混合照明复用傅里叶叠层显微术
Hybrid-illumination multiplexed Fourier ptychographic microscopy with robust aberration correction
AI总结：
针对傅里叶叠层显微术采集慢、复用方案像差校正不足的问题，提出结合解析像差提取与混合照明的HMFPM方法，仅用20次测量实现4倍于相干衍射极限的分辨率，兼具鲁棒像差校正与高通量成像能力。
摘要：
傅里叶叠层显微术（FPM）是一种强大的计算成像模态，可对生物医学样本实现高空间带宽积成像。然而，由于需要进行序列测量，其数据采集速度缓慢，限制了该技术的推广应用。已有研究提出复用FPM策略，通过同时激活多个LED来加速成像，但这类方法通常需要精细的参数调优，且缺乏有效的像差校正，容易导致图像质量下降。为解决这些局限，我们提出混合照明复用傅里叶叠层显微术（HMFPM），该方法将解析像差提取能力与复用照明的高效性相结合。具体而言，HMFPM采用混合照明策略，以及一套结合解析方法与优化方法的定制化重建算法。这种混合策略大幅减少了所需的测量次数，同时确保了鲁棒的像差校正与稳定的收敛性。我们通过实验证明，HMFPM在1.77×1.77平方毫米的视场内，仅需20次测量即可实现1.08微米的分辨率，较系统的相干衍射极限提升了4倍。HMFPM在多种像差条件下仍保持鲁棒性，具备最高84微米的数字重聚焦能力，可有效校正全切片病理成像中的场相关像差与扫描引入的像差。这些结果表明，HMFPM是一种适用于生物与生物医学成像的实用、高通量且无像差的成像解决方案。
英文摘要：
Fourier ptychographic microscopy (FPM) is a powerful computational imaging modality that achieves high space-bandwidth product imaging for biomedical samples. However, its adoption is limited by slow data acquisition due to the need for sequential measurements. Multiplexed FPM strategies have been proposed to accelerate imaging by activating multiple LEDs simultaneously, but they typically require careful parameter tuning, and their lack of effective aberration correction makes them prone to image degradation. To address these limitations, we introduce hybrid-illumination multiplexed Fourier ptychographic microscopy (HMFPM), which integrates analytic aberration extraction capability with the efficiency of multiplexed illumination. Specifically, HMFPM employs a hybrid illumination strategy and a customized reconstruction algorithm with analytic and optimization methods. This hybrid strategy substantially reduces the number of required measurements while ensuring robust aberration correction and stable convergence. We demonstrate that HMFPM achieves 1.08 micrometers resolution, representing a 4-fold enhancement over the system's coherent diffraction limit, across a 1.77x1.77 millimeter square field of view using 20 measurements. HMFPM remains robust under diverse aberrations, providing up to 84 micrometers digital refocusing capability, and effectively corrects both field-dependent and scanning-induced aberrations in whole-slide pathology imaging. These results establish HMFPM as a practical, high-throughput, and aberration-free solution for biological and biomedical imaging.
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