FPM-WSI：通过特征域反向衍射实现傅里叶叠层全切片成像
FPM-WSI: Fourier ptychographic whole slide imaging via feature-domain backdiffraction
AI总结：
针对FPM分块拼接导致的颜色不一致与伪影问题，提出特征域反向衍射框架，实现全视场无拼接重建、像差补偿、低频谱重叠恢复与盲自动重对焦。
摘要：
傅里叶叠层显微术（FPM）以高通量计算成像为特征，理论上为空间分辨率与视场（FOV）之间的权衡提供了一种巧妙的解决方案，在数字病理学应用中具有广阔前景。然而，由于渐晕效应，目前分块重建后再拼接已成为不可避免的流程。拼接图像往往在不同图像片段之间呈现颜色不一致，甚至出现拼接伪影。针对这一问题，我们提出了一种基于特征域反向衍射的计算框架，以实现全视场、无拼接的FPM重建。与在图像域建立损失函数的传统算法不同，本方法在特征域中构建损失函数，由特征提取器提取图像的有效信息，从而绕过渐晕效应。随后，基于模型参数估计得到的预测图像与实际采集图像之间的特征域误差，通过光学系统进行数字反向衍射，用于复振幅重建和像差补偿。通过大量仿真和实验，该方法有效消除了渐晕伪影，并降低了对精确获知照明位置的要求。我们还发现，该方法在恢复具有较低频谱重叠率的数据，以及在无需预先获知离焦距离的情况下实现自动盲数字重对焦方面具有巨大潜力。
英文摘要：
Fourier ptychographic microscopy (FPM), characterized by high-throughput computational imaging, theoretically provides a cunning solution to the trade-off between spatial resolution and field of view (FOV), which has a promising prospect in the application of digital pathology. However, block reconstruction and then stitching has currently become an unavoidable procedure due to vignetting effects. The stitched image tends to present color inconsistency in different image segments, or even stitching artifacts. In response, we reported a computational framework based on feature-domain backdiffraction to realize full-FOV, stitching-free FPM reconstruction. Different from conventional algorithms that establish the loss function in the image domain, our method formulates it in the feature domain, where effective information of images is extracted by a feature extractor to bypass the vignetting effect. The feature-domain error between predicted images based on estimation of model parameters and practically captured images is then digitally diffracted back through the optical system for complex amplitude reconstruction and aberration compensation. Through massive simulations and experiments, the method presents effective elimination of vignetting artifacts, and reduces the requirement of precise knowledge of illumination positions. We also found its great potential to recover the data with a lower overlapping rate of spectrum and to realize automatic blind-digital refocusing without a prior defocus distance.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
