蛋白质组学策略中电泳分离的能力和局限性
Power and limitations of electrophoretic separations in proteomics strategies
摘要：
蛋白质组学可以定义为对蛋白质的大规模分析。由于生物系统的复杂性，需要在质谱分析之前串联各种分离技术。这些处理蛋白质或肽的技术可以依赖于色谱法或电泳法。在这篇评论中，电泳技术正在审查。他们的原则被召回，他们的应用肽和蛋白质分离的介绍和批判性的讨论。此外，凝胶电泳特有的和与质谱相互作用的特征（即，电泳后的蛋白质检测，以及从凝胶片到肽溶液的过程）也进行了讨论。
英文摘要：
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis. In this review, the electrophoretic techniques are under scrutiny. Their principles are recalled, and their applications for peptide and protein separations are presented and critically discussed. In addition, the features that are specific to gel electrophoresis and that interplay with mass spectrometry (i.e., protein detection after electrophoresis, and the process leading from a gel piece to a solution of peptides) are also discussed.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
