聚丙烯酰胺凝胶中蛋白质的银染
Silver staining of proteins in polyacrylamide gels
摘要：
银染色用于在聚丙烯酰胺凝胶上电泳分离后检测蛋白质。它结合了出色的灵敏度（在低纳克范围内）与使用非常简单和廉价的设备和化学品。它与下游处理兼容，例如蛋白质消化后的质谱分析。银染的顺序是蛋白质固定，然后敏化，然后银浸渍，最后图像显影。银染色的几种变体在此描述，其可以在电泳分离结束后2 h至1 d的时间范围内完成。一旦完成，染色剂可稳定数周。
英文摘要：
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is compatible with downstream processing, such as mass spectrometry analysis after protein digestion. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 d after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks.
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