2DE凝胶中蛋白质的银染
Silver Staining of Proteins in 2DE Gels
摘要：
银染色检测聚丙烯酰胺凝胶电泳分离后的蛋白质。它的主要优点是灵敏度高（在低纳克范围内），使用非常简单和便宜的设备和化学品。银染的顺序是蛋白质固定，然后敏化，然后银浸渍，最后图像显影。这里描述了银染色的几种变体，其可以在电泳分离结束后2小时至1天的时间范围内完成。一旦完成，染色剂可稳定数周。
英文摘要：
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 day after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks.
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