Sweet silver：使用醛糖作为显影剂的无甲醛银染，与质谱法的兼容性增强
Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry
摘要：
电泳后的蛋白质检测方法必须是灵敏的、均质的，并且不损害通过MS的蛋白质的下游分析。速度、低成本和用户友好性也是有利的特征。银染色结合了许多这些特征，但其与MS的兼容性有限。我们在这里描述的是一种新的银染变体，它完全不含甲醛。碱性硼酸盐缓冲液中的还原糖用作显影剂。在保留银染优点的同时，该方法在MALDI PMF和LC/ESI/MS/MS中与MS的兼容性方面显示出更好的性能。
英文摘要：
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features, but its compatibility with MS is limited. We describe here, a new variant of silver staining that is completely formaldehyde-free. Reducing sugars in alkaline borate buffer are used as developers. While keeping the benefits of silver staining, this method is shown to afford a much better performance in terms of compatibility with MS, both in PMF by MALDI and in LC/ESI/MS/MS.
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