膜蛋白质全变性双向电泳技术的重要进展
Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update
摘要：
蛋白质组学分析的质量和容易性取决于所使用的分析工具的性能，并且因此取决于用于对复杂蛋白质样品进行去卷积的蛋白质分离工具的性能。在蛋白质样品中，膜蛋白是最困难的样品类别之一，因为它们的疏水性和嵌入在脂质双层中。本文综述了仅分离变性蛋白质的双向电泳技术在膜蛋白分离方面的最新进展。传统的二维方法，即，将在第一维中使用IEF的方法与在两个维度中仅使用区带电泳的方法进行比较，即，在阳离子或阴离子洗涤剂存在下电泳。对这两种类型方法的总体性能和应用领域进行了严格审查，并对该领域的未来前景进行了审查。
英文摘要：
The quality and ease of proteomics analysis depends on the performance of the analytical tools used, and thus of the performances of the protein separation tools used to deconvolute complex protein samples. Among protein samples, membrane proteins are one of the most difficult sample classes, because of their hydrophobicity and embedment in the lipid bilayers. This review deals with the recent progresses and advances made in the separation of membrane proteins by 2-DE separating only denatured proteins. Traditional 2-D methods, i.e., methods using IEF in the first dimension are compared to methods using only zone electrophoresis in both dimensions, i.e., electrophoresis in the presence of cationic or anionic detergents. The overall performances and fields of application of both types of method is critically examined, as are future prospects for this field.
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