双向电泳前蛋白质增溶的去污剂和离液剂
Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis
摘要：
由于二维电泳分离完整蛋白质的复杂混合物的出色能力，将其应用于所有类型的蛋白质，包括疏水蛋白和膜蛋白，将是有利的。不幸的是，差的溶解度阻碍了这些分子的分析。由于这些问题主要出现在提取和等电聚焦步骤中，因此解决方案是在等电聚焦期间占主导地位的条件下改善蛋白质溶解度。本章介绍了使用离液剂和新型洗涤剂，以提高蛋白质的溶解度在样品提取和等电聚焦，并讨论了这些化合物的贡献，以提高膜蛋白的蛋白质组学分析。
英文摘要：
Because of the outstanding ability of two-dimensional electrophoresis to separate complex mixtures of intact proteins, it would be advantageous to apply it to all types of proteins, including hydrophobic and membrane proteins. Unfortunately, poor solubility hampers the analysis of these molecules. As these problems arise mainly in the extraction and isoelectric focusing steps, the solution is to improve protein solubility under the conditions prevailing during isoelectric focusing. This chapter describes the use of chaotropes and novel detergents to enhance protein solubility during sample extraction and isoelectric focussing, and discusses the contribution of these compounds to improving proteomic analysis of membrane proteins.
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