线粒体蛋白质组学：全线粒体提取物的双向电泳分析
Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis
摘要：
线粒体是复杂的细胞器，其蛋白质组学分析需要多种技术的结合。本章的重点是首先从培养的哺乳动物细胞中制备线粒体，然后用二维电泳（2DE）分离线粒体蛋白，显示了一些调整，以提高线粒体蛋白的分辨率的经典技术。这涵盖了蛋白质溶解、电泳部分本身和凝胶上的蛋白质检测，这使得与蛋白质鉴定部分的接口依赖于质谱法。
英文摘要：
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial proteins with two-dimensional electrophoresis (2DE), showing some adjustment over the classical techniques to improve resolution of the mitochondrial proteins. This covers both the protein solubilization, the electrophoretic part per se, and the protein detection on the gels, which makes the interface with the protein identification part relying on mass spectrometry.
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