用于治疗和预防骨感染的多功能pH敏感性三维支架
Multifunctional pH sensitive 3D scaffolds for treatment and prevention of bone infection
摘要：
已经制备了多功能治疗性3D支架。这些生物材料能够破坏S。金黄色葡萄球菌生物膜，并允许骨再生在同一时间。本研究的重点是设计pH敏感的三维分级介孔-大孔支架的基础上MGHA纳米复合材料形成的介孔结构的玻璃状网络与嵌入羟基磷灰石纳米粒子，其介孔已加载左氧氟沙星作为抗菌剂。这些3D平台表现出受控的和pH依赖性的左氧氟沙星释放，在生理pH（7.4）下随时间持续，并且在感染pH（6.7和5.5）下显著增加，这是由于不同的左氧氟沙星物质与二氧化硅基质之间的不同相互作用速率。这些3D系统能够抑制S。金黄色葡萄球菌生长并破坏细菌生物膜，而对人成骨细胞没有细胞毒性作用，并允许前成骨细胞在其表面上充分定植和分化。这些发现表明这些分级MGHA纳米复合支架用于治疗和预防骨感染的有希望的应用。
英文摘要：
Multifunctional-therapeutic 3D scaffolds have been prepared. These biomaterials are able to destroy the S. aureus bacteria biofilm and to allow bone regeneration at the same time. The present study is focused on the design of pH sensitive 3D hierarchical meso-macroporous scaffolds based on MGHA nanocomposite formed by a mesostructured glassy network with embedded hydroxyapatite nanoparticles, whose mesopores have been loaded with levofloxacin as antibacterial agent. These 3D platforms exhibit controlled and pH-dependent levofloxacin release, sustained over time at physiological pH (7.4) and notably increased at infection pH (6.7 and 5.5), which is due to the different interaction rate between diverse levofloxacin species and the silica matrix. These 3D systems are able to inhibit the S. aureus growth and to destroy the bacterial biofilm without cytotoxic effects on human osteoblasts and allowing an adequate colonization and differentiation of preosteoblastic cells on their surface. These findings suggest promising applications of these hierarchical MGHA nanocomposite scaffolds for the treatment and prevention of bone infection.
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