具有促血管生成和抗菌性能的含纳米颗粒三维多孔骨支架的制备
Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties
摘要：
通过GELPOR3D方法制备了基于琼脂糖和纳米晶磷灰石的3D多孔支架，这两种结构组分充当临时矿化的细胞外基质。这种成形技术允许在制造过程中在支架内引入热不稳定分子。一种血管生成蛋白，血管内皮生长因子，和抗生素，头孢氨苄，装载在介孔二氧化硅纳米粒子，包括设计多功能支架骨重建。两种分子的双重释放显示出在卵外生长的鸡胚中的促血管生成行为，同时提供能够抑制金黄色葡萄球菌细菌生长的抗生素局部浓度。从这个意义上说，不同的释放模式，监测紫外光谱，可以定制作为一个功能的头孢氨苄加载策略。支架表面的特征在于高亲水性，如通过接触角测量所确定的，这有利于前成骨细胞的粘附和增殖。
英文摘要：
3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method. This shaping technology allows the introduction of thermally-labile molecules within the scaffolds during the fabrication procedure. An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction. The dual release of both molecules showed a pro-angiogenic behaviour in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth. In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy. The scaffold surface was characterized by a high hydrophilicity, as determined by contact angle measurements, that facilitated the adhesion and proliferation of preosteoblastic cells.
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