Non-invasive detection of microvascular alterations in deep tissues in vivo provides critical information for clinical diagnosis and evaluation of a broad-spectrum of pathologies. Recently, the emergence of super-resolution ultrasound localization microscopy (ULM) offers new possibilities for clinical imaging of microvasculature at capillary level. Currently, the clinical utility of ULM on clinical ultrasound scanners is hindered by the technical limitations, such as long data acquisition time, and compromised tracking performance associated with low imaging frame-rate. Here we present an in-human ULM on a high frame-rate (HFR) clinical ultrasound scanner to achieve super-resolution microvessel imaging using a short acquisition time (<10s). Ultrasound MB data were acquired from different human tissues, (liver, kidney, pancreatic, and breast tumor) using an HFR clinical scanner. By leveraging the HFR and advanced processing techniques including sub-pixel motion registration, MB signal separation, and Kalman filter-based tracking, MBs can be robustly localized and tracked for successful ULM under the circumstances of relatively high MB concentration and limited data acquisition time in humans. Subtle morphological and hemodynamic information were demonstrated on data acquired with single breath-hold and free-hand scanning. Compared with contrast-enhanced power Doppler generated based on the same MB dataset, ULM showed a 5.7-fold resolution improvement in a vessel, and provided a wide-range flow speed measurement that is Doppler angle-independent. This study demonstrated the feasibility of ultrafast in-human ULM in various human tissues based on a clinical scanner that supports HFR imaging, and showed a great potential for the implementation of super-resolution ultrasound microvessel imaging in a myriad of clinical applications involving microvascular abnormalities and pathologies.
Accurate and Generalizable Quantitative Scoring of Liver Steatosis from Ultrasound Images via Scalable Deep Learning
PAII Inc.（PAII 公司）；Chang Gung Memorial Hospital, Linkou Medical Center（长庚纪念医院林口医学中心）；Institute of Molecular and Genomic Medicine, National Health Research Institutes（国家卫生研究院分子与基因组医学研究所）；Ping An Insurance Group（平安保险集团）
Efficient Image Registration for Ultrasound Localization Microscopy by Obtaining Gradients via Integration Across Iterations
Imperial College London（帝国理工学院）；State Key Laboratory of Robotics and Systems, Harbin Institute of Technology（哈尔滨工业大学机器人技术与系统国家重点实验室）；The University of Melbourne（墨尔本大学）
Ultrafast 3-D Super Resolution Ultrasound using Row-Column Array specific Coherence-based Beamforming and Rolling Acoustic Sub-aperture Processing: In Vitro, In Vivo and Clinical Study
Simultaneous 3D co-registered perfusion and oxygenation with ULM, photoacoustic imaging, and a planar matrix array
Sorbonne Université（索邦大学）；CNRS（法国国家科学研究中心）；Inserm（法国国家健康与医学研究院）；Laboratoire d’Imagerie Biomédicale, LIB（生物医学成像实验室）；Université Paris Cité（巴黎西岱大学）；Centre de Recherche des Cordeliers（科尔德洛耶研究中心）；Institut Cochin（科尚研究所）；Department of Nuclear Medicine, Hôpital Pitié Salpêtrière, APHP（皮蒂埃-萨尔佩特里埃医院核医学科）；PARCC（PARCC研究中心）；Service de Radiologie, AP-HP, Hôpital européen Georges Pompidou（乔治·蓬皮杜欧洲医院放射科）
Super-Resolution Posterior Ocular Microvascular Imaging Using 3-D Ultrasound Localization Microscopy With a 32X32 Matrix Array
Southern California, Los Angeles, CA, USA（南加州）；USC Roski Eye Institute（南加州大学罗斯基眼科研究所）；Department of Radiology, Mayo Clinic, College of Medicine and Science（梅奥诊所医学院放射系）；University of Rochester, MN, USA（罗切斯特大学）
Inverse Problem Approach to Aberration Correction for in vivo Transcranial Imaging Based on a Sparse Representation of Contrast-enhanced Ultrasound Data
Polytechnique Montréal（蒙特利尔高等理工学院）；Université de Montréal（蒙特利尔大学）；Montreal Heart Institute（蒙特利尔心脏研究所）
Deep-Learning Based Super-Resolution Functional Ultrasound Imaging of Transient Brain-Wide Neurovascular Activity on a Microscopic Scale
College of Biomedical Engineering, Fudan University（复旦大学 biomedical 工程学院）；Department of Neurosurgery, Huashan Hospital, Institutes for Translational Brain Research, Fudan University（复旦大学华山医院神经外科、转化脑研究院）；School of Psychology, Institute of Chinese Cultural Psychology, Shanghai Jiao Tong University（上海交通大学心理学学院、中国文化心理研究所）
Automatic Aberration Correction for Transcranial Functional and Super-Resolution Ultrasound Imaging in Rodents and Nonhuman Primates
Polytechnique Montréal（蒙特利尔高等工程学院）；Université de Montréal（蒙特利尔大学）；Centre interdisciplinaire de recherche sur le cerveau et l’apprentissage (CIRCA)（大脑与学习跨学科研究中心）；Montreal Heart Institute（蒙特利尔心脏研究所）
Determining the utility of ultrafast nonlinear contrast enhanced and super resolution ultrasound for imaging microcirculation in the human small intestine
