使用平板扫描仪的千兆像素显微镜
Gigapixel microscopy using a flatbed scanner
摘要：
具有非常宽的视场（FOV）的显微镜成像系统在生物医学应用中是高度寻求的。在本文中，我们报告了一个宽视场显微成像系统，使用低成本的扫描仪和闭路电视（CCTV）镜头。我们表明，这样的成像系统是能够捕获一个10毫米 * 7.5毫米的FOV图像与0.77微米的分辨率，导致在整个图像（26400像素 * 20400像素）的0.54千兆像素（109像素）。通过对美国空军分辨率目标和孔阵目标的成像，表征了该系统的分辨率和视场曲线。通过使用这样的系统获取病理载玻片的1.6千兆像素显微图像（0.54千兆像素，具有3种颜色）用于应用演示。
英文摘要：
Microscopy imaging systems with a very wide field-of-view (FOV) are highly sought in biomedical applications. In this paper, we report a wide FOV microscopy imaging system that uses a low-cost scanner and a closed-circuit-television (CCTV) lens. We show that such an imaging system is capable to capture a 10 mm * 7.5 mm FOV image with 0.77 micron resolution, resulting in 0.54 gigapixels (109 pixels) across the entire image (26400 pixels * 20400 pixels). The resolution and field curve of the proposed system were characterized by imaging a USAF resolution target and a hole-array target. A 1.6 gigapixel microscopy image (0.54 gigapixel with 3 colors) of a pathology slide was acquired by using such a system for application demonstration.
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