结合ULM、光声成像与平面矩阵阵列实现三维共配准的灌注与氧合同步成像
Simultaneous 3D co-registered perfusion and oxygenation with ULM, photoacoustic imaging, and a planar matrix array
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中文总结 AI 辅助
本研究开发了基于平面超声矩阵阵列的双模态成像平台，同步实现共配准的三维组织氧合与微血管灌注成像，经体外仿体与小鼠体内验证，为相关生物医学研究奠定基础。
中文摘要 AI 辅助
研究背景：组织氧合与微血管灌注的联合评估可为多种生物医学应用的血管功能研究提供重要信息。多光谱光声成像可评估血液氧合，而超声定位显微镜（ULM）能实现微血管与血液灌注的亚衍射可视化。本研究将这两种互补模态结合，同步生成共配准的三维血液氧合与灌注图谱。方法：光声成像与ULM均为超声技术，研究人员开发了一种成像平台，采用单一平面超声矩阵阵列整合两种模态，该阵列是用于三维ULM的最先进阵列。该双模态平台先通过仿体血管进行体外验证，再在小鼠体内进行验证。结果：体外注射造影剂的管道双模态成像显示共配准精度达20μm，且呈现互补的结构与功能信息；多光谱光声成像仅用5个光波长，实现了生理范围（60%-95%）内血氧饱和度测量，精度达5%；对具有已知血管解剖结构的健康小鼠组织的体内成像，进一步证明了该平台联合表征血液氧合与微血管灌注的能力。意义：本研究通过实验验证了采用平面超声阵列的双模态光声成像-ULM方法，表征了该平台的功能成像性能，并明确了该阵列配置在光声成像中固有的有限视角伪影。这些发现为未来在小鼠模型研究中采用该平台及推进这一有前景的双模态方法奠定了基础。
英文摘要
Objective. Joint assessment of tissue oxygenation and microvascular perfusion could offer valuable insights into vascular function across a wide range of biomedical applications. Multispectral photoacoustic imaging enables the evaluation of blood oxygenation, while ultrasound localization microscopy provides sub-diffraction visualization of the microvasculature and blood perfusion. Here, we combine these two complementary modalities to simultaneously generate co-registered, volumetric maps of blood oxygenation and perfusion. Approach. Photoacoustic imaging and ultrasound localization microscopy are both ultrasound-based techniques. We developed an imaging platform that integrates the two modalities using a single planar ultrasonic matrix array, a state-of-the-art array for 3D ultrasound localization microscopy. The bimodal platform was validated in vitro using vessel-mimicking phantoms, then in vivo in mice. Main results. In vitro bimodal images of tubes injected with contrast agents demonstrated a coregistration accuracy of 20 $μ$m and revealed complementary structural and functional information. Multispectral photoacoustic imaging achieved oxygen saturation measurements spanning the physiological range (60-95 %) with 5 % accuracy using only five optical wavelengths. In vivo imaging of healthy mouse tissues with known vascular anatomy further demonstrated the ability of the proposed platform to jointly characterize blood oxygenation and microvascular perfusion. Significance. This work experimentally validates a bimodal photoacoustic imaging-ultrasound localization microscopy approach using a planar ultrasound array. We characterized the functional imaging performance of this platform and identified limited-view artifacts inherent to this array configuration in photoacoustic imaging. These findings establish a foundation for adopting the platform in future studies of murine models and for advancing this promising bimodal approach.
