arXivDaily arXiv每日学术速递 周一至周五更新
arXiv 1707.04545physics.optics
收藏

Multimodal Nonlinear Microscope based on a Compact Fiber-format Laser Source

Francesco Crisafi, Vikas Kumar, Antonio Perri, Marco Marangoni, Giulio Cerullo, Dario Polli

英文摘要:

We present a multimodal non-linear optical (NLO) laser-scanning microscope, based on a compact fiber-format excitation laser and integrating coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and two-photon-excitation fluorescence (TPEF) on a single platform. We demonstrate its capabilities in simultaneously acquiring CARS and SRS images of a blend of 6-μm poly(methyl methacrylate) beads and 3-μm polystyrene beads. We then apply it to visualize cell walls and chloroplast of an unprocessed fresh leaf of Elodea aquatic plant via SRS and TPEF modalities, respectively. The presented NLO microscope, developed in house using off-the-shelf components, offers full accessibility to the optical path and ensures its easy re-configurability and flexibility.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

↑