arXivDaily arXiv每日学术速递 周一至周五更新
arXiv 2108.08440physics.optics
收藏

使用快速宽调谐光纤laser的多窗口SRS成像

Multi-window SRS imaging using a rapid widely tunable fiber laser

Hongli Ni, Peng Lin, Yifan Zhu, Meng Zhang, Yuying Tan, Yuewei Zhan, Ji-Xin Cheng

摘要:

光谱受激拉曼散射(SRS)成像已成为一个有用的工具,发现广泛的应用。然而,更广泛的采用是由庞大的和环境敏感的固态光学参量振荡器(OPO)在当前的SRS显微镜阻碍。此外,由于固态OPO的缓慢波长调谐速度,跨C-H、C-D和指纹拉曼区域的化学信息多窗口SRS成像具有挑战性。在这项工作中,我们提出了一个多窗口SRS成像系统的基础上,紧凑和强大的光纤激光器具有快速和广泛的调谐能力。针对光纤激光器固有的相对强度噪声,我们实现了自动平衡检测,使受激拉曼损耗成像的信噪比提高了23倍。我们展示了真菌,癌细胞和秀丽隐杆线虫在C-H,C-D和指纹拉曼窗口的高质量SRS代谢成像。我们的结果展示了紧凑的多窗口SRS系统的广泛应用的潜力。

英文摘要:

Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding a broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally-sensitive solid-state optical parametric oscillator (OPO) in current SRS microscope. Moreover, chemically-informative multi-window SRS imaging across C-H, C-D and fingerprint Raman regions is challenging due to the slow wavelength tuning speed of the solid-state OPO. In this work, we present a multi-window SRS imaging system based on a compact and robust fiber laser with rapid and widely tuning capability. To address the relative intensity noise intrinsic to fiber laser, we implemented auto-balanced detection which enhances the signal-to-noise ratio of stimulated Raman loss imaging by 23 times. We demonstrate high-quality SRS metabolic imaging of fungi, cancer cells, and Caenorhabditis elegans across the C-H, C-D and fingerprint Raman windows. Our re-sults showcase the potential of the compact multi-window SRS system for a broad range of applications.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

↑