脑组织纤维光度学信号量的分析与实验测量
Analytical and empirical measurement of fiber photometry signal volume in brain tissue
摘要：
纤维光度法允许监测行为动物神经活动的荧光指标。光纤通常用于激发和收集来自遗传编码的钙指示剂的荧光，所述遗传编码的钙指示剂由感兴趣的回路中的神经元的子集表达。然而，缺乏对收集信号的大脑体积以及这如何取决于光纤特性的定量理解。在这里，我们分析模型和实验测量的光发射和收集领域的光纤在溶液和散射组织，提供了一个全面的表征光纤光度法常用的光纤。由于光度信号取决于激发和收集效率，一个组合的共聚焦/2-光子显微镜的开发，以评估这些参数独立。我们发现，80%的有效信号来自从光纤面延伸约200 μ m的10^5-10^6 μ m3体积，因此允许对光纤光度法进行的测量进行空间解释。
英文摘要：
Fiber photometry permits monitoring fluorescent indicators of neural activity in behaving animals. Optical fibers are typically used to excite and collect fluorescence from genetically-encoded calcium indicators expressed by a subset of neurons in a circuit of interest. However, a quantitative understanding of the brain volumes from which signal is collected and how this depends on the properties of the optical fibers are lacking. Here we analytically model and experimentally measure the light emission and collection fields for optical fibers in solution and scattering tissue, providing a comprehensive characterization of fibers commonly employed for fiber photometry. Since photometry signals depend on both excitation and collection efficiency, a combined confocal/2-photon microscope was developed to evaluate these parameters independently. We find that the 80% of the effective signal arises from a 10^5-10^6 um3 volume extending ~200 um from the fiber face, and thus permitting a spatial interpretation of measurements made with fiber photometry.
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