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arXiv 2004.10352q-bio.NC
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大鼠坐骨神经诱发神经活动的光学相干断层成像

Optical coherence tomography imaging of evoked neural activity in sciatic nerve of rat

James Hope, Matthew Goodwin, Frederique Vanholsbeeck

摘要:

重要性:使用光学相干断层扫描(OCT)对有髓鞘组织中的神经活动进行成像为外周和中枢神经系统中的功能成像创造了新的可能性。目的:研究离体大鼠坐骨神经诱发神经活动后OCT图像的变化。方法:用扫描源偏振敏感OCT系统对大鼠周围神经进行M扫描,同时用神经袖带进行神经电记录。在总共10名受试者中:3名无刺激(对照),3名有爪刺激,4名有神经刺激。计算每名受试者和溶液中镜子和微球的参考样品的OCT信号强度、相位延迟、相位和频谱的变化。结果如下:在3个爪刺激和2个神经刺激受试者中观察到的强度变化以及在2个爪刺激受试者中观察到的频谱振幅变化高于参考噪声水平,并且在时间上与神经活动期间离子电流引起的渗透性肿胀一致。结论:由渗透性肿胀产生的光散射变化,以前在鱿鱼和螃蟹神经中被描述,也被认为发生在有髓纤维中,其程度可以使用OCT检测到

英文摘要:

Significance: Imaging neural activity in myelinated tissue using optical coherence tomography (OCT) creates new possibilities for functional imaging in the peripheral and central nervous systems. Aim: To investigate changes in OCT images in response to evoked neural activity in sciatic nerve of rat in vitro. Approach: M-scans were obtained on peripheral nerves of rat using a swept source polarisation sensitive OCT system, while a nerve cuff acquired electrical neural recordings. From a total of 10 subjects: 3 had no stimulation (controls), 3 had paw stimulation, and 4 had nerve stimulation. Changes in the OCT signal intensity, phase retardation, phase, and frequency spectra were calculated for each subject and reference samples of a mirror and microspheres in solution. Results: Observed changes in intensity in 3 paw stimulation and 2 nerve stimulation subjects and changes in frequency spectra amplitude in 2 paw stimulation subjects were above the reference noise level and were temporally consistent with osmotic swelling from ion currents during neural activity. Conclusion: Light scattering changes produced by osmotic swelling, which have previously been characterised in squid and crab nerve, are also thought to occur in myelinated fibres on a scale which is detectable using OCT

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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