波动压力辅助噬菌体排出DNA
Fluctuation Pressure Assisted Ejection of DNA From Bacteriophage
摘要：
在模型计算中讨论了在紧密包装的DNA从蛋白质衣壳壳弹射之前，热压力波动的作用。在排出前的平衡状态下，我们假设DNA多次折叠成一束平行的片段，以最小的自由能形成平衡构象，紧紧地压在衣壳内壁上。使用一个典型的合奏温度T，我们计算内部压力波动对一个缓慢移动或静态的衣壳地幔的弹性连续模型的折叠DNA束。据发现，波动压力的衣壳内壁上的纵向声振动的束中的热激发可能有均方根值，这是几十个大气压的典型小噬菌体尺寸。比较与λ噬菌体具有不同的碱基对长度和总基因组喷射压力的三个突变体的测量数据。
英文摘要：
The role of thermal pressure fluctuation excited within tightly packaged DNA prior to ejection from protein capsid shells is discussed in a model calculation. At equilibrium before ejection we assume the DNA is folded many times into a bundle of parallel segments that forms an equilibrium conformation at minimum free energy, which presses tightly against internal capsid walls. Using a canonical ensemble at temperature T we calculate internal pressure fluctuations against a slowly moving or static capsid mantle for an elastic continuum model of the folded DNA bundle. It is found that fluctuating pressure on the capsid internal wall from thermal excitation of longitudinal acoustic vibrations in the bundle may have root-mean-square values which are several tens of atmospheres for typically small phage dimensions. Comparisons are given with measured data on three mutants of lambda phage with different base pair lengths and total genome ejection pressures.
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