We study the dynamics of intracellular calcium oscillations in the presence of proteins that bind calcium on multiple sites and that are generally believed to act as passive calcium buffers in cells. We find that multisite calcium-binding proteins set a sharp threshold for calcium oscillations. Even with high concentrations of calcium-binding proteins, internal noise, which shows up spontaneously in cells in the process of calcium wave formation, can lead to self-oscillations. This produces oscillatory behaviors strikingly similar to those observed in real cells. In addition, for given intracellular concentrations of both calcium and calcium-binding proteins the regularity of these oscillations changes and reaches a maximum as a function noise variance, and the overall system dynamics displays stochastic coherence. We conclude that calcium-binding proteins may have an important and active role in cellular communication.
Multi class intracellular protein targeting predictions in diatoms and other algae with complex plastids: ASAFind 2.0
Biology Centre, Institute of Parasitology, Czech Academy of Sciences（捷克科学院寄生虫学研究所生物学中心）；Faculty of Science, University of South Bohemia（南波希米亚大学理学院）；School of Oceanography, University of Washington（华盛顿大学海洋学院）
Classification of Intracellular Protein Patterns from Reactive Equilibria
Ludwig-Maximilians-Universität München（慕尼黑大学）；Arnold Sommerfeld Center for Theoretical Physics and Center for NanoScience（阿诺德·索末菲理论物理中心与纳米科学中心）；University of California Santa Barbara（加州大学圣塔芭芭拉分校）；Kavli Institute for Theoretical Physics（卡弗里理论物理研究所）；Max Planck School Matter to Life（马克斯·普朗克物质与生活学院）
真核细胞的发展已经通过自然选择进行了优化，以服从信使蛋白的最大细胞内流量。这又意味着关于靶核孔复合物（NPR）的角位置的最大Fisher信息。细胞被简单地建模为球形，细胞膜（CM）直径为10微米，同心核膜（NM）直径为6微米。NM含有约3000个核孔复合物（NPC）。发育需要信使配体从CM-NPC-DNA靶结合位点移动。配体通过磷酸化获得负电荷，在牛顿轨迹上穿过细胞质朝向带正电荷的NPC（利用正核定位序列）。CM-NPC通道服从NPC处的最大化平均蛋白质通量F和Fisher信息I，对于环境扰动具有一阶delta I = 0和近似二阶delta I = 0稳定性。它的许多预测都得到了证实，包括1-4个蛋白质的蛋白质途径占主导地位，EGFR蛋白的大小为4 nm，通量值F =10^16个蛋白质/m2·s。在进入细胞核后，每个蛋白质最终以最大概率将其配体信息传递到DNA靶位点，即最大Kullback-Liebler熵HKL。在光滑度极限下，HKL接近IDNA/2，因此总的CM-NPC-DNA通道服从最大Fisher I。因此，最大信息接近非平衡，这是生命的一个条件。
All-optical intracellular thermal profiling using nanodiamond-based "thermal radar"
The University of Hong Kong（香港大学）；Institute for Protein Research, The University of Osaka（大阪大学蛋白质研究所）；School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong（香港大学生物医学科学院医学院）
A viscoelastic theory for ultrasound-induced intracellular streaming
Institute for Theoretical Physics, Heidelberg University（海德堡大学理论物理研究所）；BioQuant, Heidelberg University（海德堡大学生物定量研究中心）；Max Planck Institute for Medical Research（马克斯·普朗克医学研究所）
