PomXYZ蛋白在细菌类核上自组织以刺激细胞分裂
The PomXYZ Proteins Self-Organize on the Bacterial Nucleoid to Stimulate Cell Division
摘要：
精确调节细胞分裂位点定位以产生正确大小和形状的子体。我们发现了一种新的策略，将FtsZ细胞动力学环定位在社会细菌黄色粘球菌的中细胞。PomX、PomY和类核苷酸结合的ParA/MinD ATP酶PomZ自组装形成大的类核苷酸相关复合物，其定位在FtsZ之前的分裂位点以直接引导和刺激分裂。PomXYZ定位是通过类核上朝向中间细胞的自组织偏置随机运动和中间细胞处的约束运动产生的。实验和理论表明，PomXYZ运动产生的扩散PomZ通量的类核到复杂的。通量差异与复合物的细胞内不对称性成比例，并转化为跨复合物的局部PomZ浓度梯度，并向更高的PomZ浓度易位。在中间细胞处，通量均衡，导致受约束的运动。基于通量的机制可能代表了细菌中大分子结构定位的一般范式。
英文摘要：
Cell division site positioning is precisely regulated to generate correctly sized and shaped daughters. We uncover a novel strategy to position the FtsZ cytokinetic ring at midcell in the social bacterium Myxococcus xanthus. PomX, PomY and the nucleoid-binding ParA/MinD ATPase PomZ self-assemble forming a large nucleoid-associated complex that localizes at the division site before FtsZ to directly guide and stimulate division. PomXYZ localization is generated through self-organized biased random motion on the nucleoid towards midcell and constrained motion at midcell. Experiments and theory show that PomXYZ motion is produced by diffusive PomZ fluxes on the nucleoid into the complex. Flux differences scale with the intracellular asymmetry of the complex and are converted into a local PomZ concentration gradient across the complex with translocation towards the higher PomZ concentration. At midcell, fluxes equalize resulting in constrained motion. Flux-based mechanisms may represent a general paradigm for positioning of macromolecular structures in bacteria.
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