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arXiv physics/0601015physics.chem-phphysics.bio-ph
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细胞大小的磷脂囊泡在水/油界面上的转运

Transporting of a Cell-Sized Phospholipid Vesicle Across Water/Oil Interface

Masahiko Hase, Ayako Yamada, Tsutomu Hamada, Kenichi Yoshikawa

摘要:

当将直径为几十微米的细胞大小的水滴置于含有磷脂的油中时,稳定的细胞大小的囊泡自发形成为具有磷脂单层的油包水磷脂乳液(W/O CE)。我们通过显微操作将由此在油相中形成的脂质囊泡转移到水相中穿过水/油界面,这表明囊泡从作为W/O CE的磷脂单层转化为双层。然后,脂质囊泡可以被转运回油相中。这种新的实验方法可能是一个有用的工具,用于创建一个模型细胞系统,它与一个微反应器,适用于作为一个微米级的生化反应场。

英文摘要:

When a cell-sized water droplet, with a diameter of several tens of micro meter, is placed in oil containing phospholipids, a stable cell-sized vesicle is spontaneously formed as a water-in-oil phospholipid emulsion (W/O CE) with a phospholipid monolayer. We transferred the lipid vesicle thus formed in the oil phase to the water phase across the water/oil interface by micromanipulation, which suggests that the vesicle is transformed from a phospholipid monolayer as W/O CE into a bilayer. The lipid vesicle can then be transported back into the oil phase. This novel experimental procedure may be a useful tool for creating a model cellular system, which, together with a microreactor, is applicable as a micrometer-scale biochemical reaction field.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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