蔗糖溶液作为研究囊泡结构的前瞻性介质：SAXS和SANS研究
Sucrose Solutions as Prospective Medium to Study the Vesicle Structure: SAXS and SANS study
摘要：
探讨了用蔗糖溶液作为介质进行DMPC囊泡和DMPC/C_（12）E_（8）混合聚集体的X射线和中子小角散射实验的可能性。蔗糖浓度对磷脂囊泡的大小和多分散性的影响进行了研究，通过互补X射线和中子散射。蔗糖溶液降低囊泡的大小和多分散性，并增加了磷脂膜和散装溶剂之间的对比度足以为X射线。与纯H2O相比，H2O中的40%蔗糖使X射线对比度增加了10倍。蔗糖浓度在30%-40%范围内为磷脂囊泡的X射线小角实验创造了最佳实验条件。
英文摘要：
The possibility to use sucrose solutions as medium for X-ray and neutron small-angle scattering experiments has been explored for dimyristoylphosphatidylcholine (DMPC) vesicles and mixed DMPC/C_(12)E_(8) aggregates. The influence of sucrose concentration on phospholipid vesicles size and polydispersity has been investigated by complimentary X-ray and neutron scattering. Sucrose solutions decreased vesicle size and polydispersity and increased a contrast between phospholipid membrane and bulk solvent sufficiently for X-rays. 40% sucrose in H2O increased X-ray contrast by up to 10 times compared to pure H2O. The range of sucrose concentration 30%-40% created the best experimental conditions for the X-ray small-angle experiment with phospholipid vesicles.
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