SAXS和SANS揭示的氧化铁负载对溶液中磁性铁蛋白结构的影响
Effect of iron oxide loading on magnetoferritin structure in solution as revealed by SAXS and SANS
摘要：
合成生物大分子磁性铁蛋白（脱铁铁蛋白），其在蛋白质壳内含有氧化铁核（脱铁铁蛋白），并具有不同的铁含量。用小角同步辐射X射线（SAXS）和中子（SANS）散射分析了它在水溶液中的结构。负载因子（LF）定义为每个蛋白质的平均铁原子数，变化高达LF=800。随着LF的增加，散射曲线的总散射强度相对增加，部分拖尾和SANS对比度变化数据中的匹配点移动。分析表明，增加的多分散性的蛋白质和相应的有效增加的磁性材料的相对含量对蛋白质部分的壳与LF的增长。在高于~150的LF下，脱铁铁蛋白壳经历结构变化，这强烈指示壳稳定性受氧化铁存在影响的事实。
英文摘要：
Synthetic biological macromolecule of magnetoferritin containing an iron oxide core inside a protein shell (apoferritin) is prepared with different content of iron. Its structure in aqueous solution is analyzed by small-angle synchrotron X-ray (SAXS) and neutron (SANS) scattering. The loading factor (LF) defined as the average number of iron atoms per protein is varied up to LF=800. With an increase of the LF, the scattering curves exhibit a relative increase in the total scattered intensity, a partial smearing and a shift of the match point in the SANS contrast variation data. The analysis shows an increase in the polydispersity of the proteins and a corresponding effective increase in the relative content of magnetic material against the protein moiety of the shell with the LF growth. At LFs above ~150, the apoferritin shell undergoes structural changes, which is strongly indicative of the fact that the shell stability is affected by iron oxide presence.
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