固体浸没荧光显微镜中高效近场光耦合的傅里叶成像研究
Fourier imaging study of efficient near-field optical coupling in solid immersion fluorescence microscopy
摘要：
我们测量了0.11-和0.22- $μ$ m直径染料掺杂聚苯乙烯微球珠在固体浸没透镜上的荧光图像和傅立叶图像，并通过实验验证了由于固体浸没荧光显微镜中有效的近场光耦合引起的强烈的角度依赖性荧光强度。结果解释与计算的发射偶极子放置在固体表面附近的发射模式进行比较，这建立了一个基本模型，高收集效率的固体浸没荧光显微镜。
英文摘要：
We measured images and Fourier images of fluorescence for 0.11- and 0.22-$μ$m-diameter dye-doped polystyrene micro-sphere beads on a solid immersion lens, and experimentally verified strongly-angle-dependent fluorescence intensities due to efficient near-field optical coupling in solid immersion fluorescence microscopy. The results are interpreted in comparison with calculated emission patterns of an emission dipole placed near a solid surface, which establish a basic model for high-collection efficiency in solid-immersion fluorescence microscopy.
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