光学分辨率下非辐射动力学的空间偏移泵浦-探测成像
Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution
AI总结：
本文提出空间偏移泵浦-探测成像（SOPPI），通过偏移泵浦与探测光束实现光热和光声波传播的高时空分辨同时成像，并开发SOPPI-PACT用于斑马鱼幼体色素分布的高精度重建。
摘要：
非辐射光热（PT）和光声（PA）过程已在成像、刺激和治疗中得到广泛应用。以高分辨率绘制PA和PT波的产生与传播对于阐明这些场如何与生物系统相互作用至关重要。为此，我们提出了空间偏移泵浦-探测成像（SOPPI）。通过将泵浦光束与探测光束在空间上偏移，SOPPI能够同时成像PA和PT波的传播，具有纳秒时间分辨率、微米空间分辨率、65 MHz检测带宽以及9.9 Pa噪声等效压力的灵敏度。我们首先绘制了光纤发射器产生的PA和PT演化过程，以及波与小鼠颅骨和脑切片相互作用的情况。对以水为吸收体的锥形光纤产生的PA波进行SOPPI成像，显示出波长依赖的产生、倏逝波产生的PA以及反向传播的声学马赫锥。最后，我们开发了一种SOPPI-PACT，以高精度和高信噪比重建斑马鱼幼体内色素分布。
英文摘要：
Nonradiative photothermal (PT) and photoacoustic (PA) processes have found widespread applications in imaging, stimulation, and therapy. Mapping the generation and propagation of PA and PT waves with resolution is important to elucidate how these fields interact with biological systems. To this end, we introduce spatial offset pump-probe imaging (SOPPI). By spatially offsetting the pump beam and the probe beam, SOPPI can image simultaneously PA and PT wave propagation with nanosecond temporal resolution, micrometer spatial resolution, 65 MHz detection bandwidth, and a sensitivity of 9.9 Pa noise equivalent pressure. We first map the PA and PT evolution from a fiber emitter, and how the wave interacting with a mouse skull and brain slices. SOPPI imaging of PA waves from a tapered fiber with water as an absorber shows a wavelength-dependent generation, evanescent wave generated PA, and back-propagated acoustic Mach Cone. At last, a SOPPI-PACT is developed to reconstruct the pigment distribution inside a zebrafish larva with high precision and signal-to-noise ratio.
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