利用光镊实现微粒进入巨型脂质囊泡
Entry of Microparticles into Giant Lipid Vesicles by Optical Tweezers
AI总结：
本研究利用光镊探究在无强结合作用下微粒穿透巨型单层囊泡脂质膜的过程，发现施加皮牛顿级外力且膜张力较低时微粒可进入，并在黏附力消失极限下揭示了膜面积储库作用及弯曲毛细长度处的最小力。
摘要：
微米或纳米级物体进入由脂质膜构成的细胞或囊泡的过程广泛存在于多种现象中，例如病毒侵入宿主细胞、微塑料污染、药物递送或生物医学成像。在此，我们研究了在缺乏强结合相互作用（例如链霉亲和素-生物素结合）的条件下，微粒穿过巨型单层囊泡脂质膜的过程。在这些条件下，我们观察到，只要施加外部皮牛顿力且膜张力相对较低，有机和无机微粒始终能够穿透进入囊泡内部。在黏附力趋于零的极限下，我们指出了膜面积储库的作用，并证明当微粒尺寸与弯曲毛细长度相当时，存在一个最小力。
英文摘要：
Entry of micro- or nano-sized objects into cells or vesicles made of lipid membranes occur in many processes such as entry of viruses in host cells, microplastics pollution, drug delivery or biomedical imaging. Here, we investigated the microparticle crossing of lipid membranes in giant unilamellar vesicles in the absence of strong binding interactions (e.g. streptavidin-biotin binding). In these conditions, we observed that organic and inorganic particles can always penetrate inside the vesicles provided that an external picoNewton force is applied and for relatively low membrane tensions. In the limit of a vanishing adhesion, we pointed out the role of the membrane area reservoir and show that a force minimum exists when the particle size is comparable to the bendocapillary length.
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