具有抗粘附两性离子表面的释锶介孔生物活性玻璃作为骨再生的高级生物材料（tissue）
Strontium-releasing mesoporous bioactive glasses with anti-adhesive zwitterionic surface as advanced biomaterials for bone tissue regeneration
摘要：
假设骨折的治疗仍然是一个具有挑战性的临床问题时，由于骨重建受损（即骨质疏松症）或感染发生并发症。这些临床需求仍然需要通过设计先进的生物材料来彻底改进现有的治疗方法，该生物材料将促进骨再生的能力与能够最小化非特异性生物分子吸附和细菌粘附的防污/抗粘附特性相结合。含锶的介孔生物活性玻璃（Sr-MBG），能够通过释放Sr 2+离子发挥促成骨作用，已被成功地功能化，以提供具有低污染能力的混合电荷表面基团。实验Sr-MBG已经通过共接枝含有氨基（氨基丙基硅烷三醇）和羧酸酯（羧乙基硅烷三醇）部分的可水解短链硅烷进行合成后改性，以实现两性离子零电荷表面，然后在质地结构性质、生物活性、细胞毒性、促成骨和低污染能力方面进行表征。结果两性化后的体外生物活性保持，以及能够释放Sr 2+离子能够诱导矿化过程。无论其大小如何，Sr-MBG颗粒在浓度高达75 μ g/mL的前成骨细胞MC 3 T3-E1中没有表现出任何细胞毒性。最后，两性离子Sr-MBG显示出相对于原始血清蛋白粘附的显著降低。这些结果在结合促成骨和抗粘附特性的纳米系统的设计中打开了有希望的未来期望。
英文摘要：
Hypothesis The treatment of bone fractures still represents a challenging clinical issue when complications due to impaired bone remodelling (i.e. osteoporosis) or infections occur. These clinical needs still require a radical improvement of the existing therapeutic approach through the design of advanced biomaterials combining the ability to promote bone regeneration with anti-fouling/anti-adhesive properties able to minimise unspecific biomolecules adsorption and bacterial adhesion. Strontium-containing mesoporous bioactive glasses (Sr-MBG), able to exert a pro-osteogenic effect by releasing Sr2+ ions, have been successfully functionalised to provide mixed-charge surface groups with low-fouling abilities. Experiments Sr-MBG have been post-synthesis modified by co-grafting hydrolysable short chain silanes containing amino (aminopropylsilanetriol) and carboxylate (carboxyethylsilanetriol) moieties to achieve a zwitterionic zero-charge surface and then characterised in terms of textural-structural properties, bioactivity, cytotoxicity, pro-osteogenic and low-fouling capabilities. Findings After zwitterionization the in vitro bioactivity is maintained, as well as the ability to release Sr2+ ions capable to induce a mineralization process. Irrespective of their size, Sr-MBG particles did not exhibit any cytotoxicity in pre-osteoblastic MC3T3-E1 up to the concentration of 75 ug/mL. Finally, the zwitterionic Sr-MBGs show a significant reduction of serum protein adhesion with respect to pristine ones. These results open promising future expectations in the design of nanosystems combining pro-osteogenic and anti-adhesive properties.
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