两性离子生物相容性介孔玻璃细菌粘附的预防
Prevention of bacterial adhesion to zwitterionic biocompatible mesoporous glasses
摘要：
本文报道了以SiO_2-CaO-P_2 O_5三元系中孔生物活性玻璃（MBG）为基础材料，采用后接枝法在其外表面分别修饰上（3-氨丙基）三乙氧基硅烷（APTES）和氨基酸赖氨酸（Lys）的新型生物活性材料（MBG-NH_2和MBG-Lys）。用有机基团的表面官能化没有破坏介孔网络，并且尽管MBG基质的高溶解度，它们的结构和纹理特性也被保留。由于外表面中存在相邻的胺和羧基，Lys的掺入赋予这些MBG材料两性离子性质。在生理pH下，这种来自锚定赖氨酸的碱性胺和羧酸基团的共存提供了零表面电荷，称为两性离子效应。这种行为可能会引起抗菌粘附的潜在应用。因此，为了评估两性离子性质对体外细菌粘附的影响，用金黄色葡萄球菌进行了研究。结果表明，与裸MBG相比，这些两性离子对在MBG表面上的有效相互作用使细菌粘附减少高达99.9%。为了测试两性离子MBGs材料作为骨移植物的适用性，在体外用MC 3 T3-E1前成骨细胞研究了它们的细胞相容性。这些发现表明，所提出的表面功能化策略为MBG材料提供了显著的抗菌粘附性能，因此使这些材料成为局部骨感染治疗的有希望的候选者。
英文摘要：
Novel materials, based on Mesoporous Bioactive Glasses (MBGs) in the ternary system SiO2-CaO-P2O5, decorated with (3-aminopropyl)triethoxysilane (APTES) and subsequently with amino acid Lysine (Lys), by post-grafting method on the external surface of the glasses (named MBG-NH2 and MBG-Lys), are reported. The surface functionalization with organic groups did not damage the mesoporous network and their structural and textural properties were also preserved despite the high solubility of MBG matrices. The incorporation of Lys confers a zwitterionic nature to these MBG materials due to the presence of adjacent amine and carboxylic groups in the external surface. At physiologic pH, this coexistence of basic amine and carboxilic acid groups from anchored Lys provided zero surface charge named zwitterionic effect. This behaviour could give rise to potential applications of antibacterial adhesion. Therefore, in order to assess the influence of zwitterionic nature in in vitro bacterial adhesion, studies were carried out with Staphylococcus aureus. It was demonstrated that the efficient interaction of these zwitterionic pairs onto the MBG surfaces reduced bacterial adhesion up to 99.9% compared to bare MBGs. In order to test the suitability of zwitterionic MBGs materials as bone grafts, their cytocompatibility was investigated in vitro with MC3T3-E1 preosteoblasts. These findings suggested that the proposed surface functionalization strategy provided MBG materials with notable antibacterial adhesion properties, hence making these materials promising candidates for local bone infection therapy.
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