氨丙基修饰介孔二氧化硅纳米粒子的特性。对主动瞄准能力的影响
Features of aminopropyl modified mesoporous silica nanoparticles. Implications on the active targeting capability
摘要：
以正硅酸乙酯（TEOS）和氨丙基三乙氧基硅烷（APTES）为原料，采用共缩聚法合成了氨丙基修饰的介孔SiO2纳米粒子MCM-41。通过修改TEOS/APTES的比例，我们已经进行了深入的表征的纳米粒子作为APTES含量的函数。表面电荷和纳米粒子的形态受到APTES的量的强烈影响，并且在APTES增加的情况下，粒子从六方变为豆状形态。此外，多孔结构也受到影响，表现为晶格参数和孔径的收缩，而增加壁厚。这些结果带来了新的见解纳米粒子的形成过程中的共缩合过程。本文提出的模型认为，TEOS和APTES与结构导向剂之间建立的不同相互作用对孔径、壁厚和颗粒形态有影响。最后，APTES是共价连接活性靶向剂如叶酸基团的优良连接体。我们假设APTES也可以在纳米颗粒的生物学行为中发挥作用。因此，已经用癌性LNCaP和非癌性前成骨细胞样MC 3 T3-E1细胞测试了纳米颗粒的内化效率。结果表明氨丙基硅烷的存在和叶酸之间的协同作用，仅对于癌性LNCaP细胞系。
英文摘要：
Aminopropyl modified mesoporous SiO2 nanoparticles, MCM-41 type, have been synthesized by the co-condensation method from tetraethylorthosilicate (TEOS) and aminopropyltriethoxysilane (APTES). By means of modifying TEOS/APTES ratio we have carried out an in-depth characterization of the nanoparticles as a function of APTES content. Surface charge and nanoparticles morphology were strongly influenced by the amount of APTES and particles changed from hexagonal to bean-like morphology insofar APTES increased. Besides, the porous structure was also affected, showing a contraction of the lattice parameter and pore size, while increasing the wall thickness. These results bring about new insights about the nanoparticles formation during the co-condensation process. The model proposed herein considers that different interactions stablished between TEOS and APTES with the structure directing agent have consequences on pore size, wall thickness and particle morphology. Finally, APTES is an excellent linker to covalently attach active targeting agents such as folate groups. We have hypothesized that APTES could also play a role in the biological behavior of the nanoparticles. So, the internalization efficiency of the nanoparticles has been tested with cancerous LNCaP and non-cancerous preosteoblast-like MC3T3-E1 cells. The results indicate a cooperative effect between aminopropylsilane presence and folic acid, only for the cancerous LNCaP cell line.
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