介孔生物活性玻璃对成骨细胞、破骨细胞和巨噬细胞的影响
Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages
摘要：
合成了一种分子组成为75 SiO2 - 20 CaO-5 P2 O 5（MBG-75 S）的介孔生物活性玻璃（MBG-75 S）。X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、氮气吸附和透射电子显微镜（TEM）研究表明，MBG-75 S具有高度有序的介孔结构，具有高的比表面积和孔隙率，这可以解释其与周围介质的高离子交换速率（主要是钙和硅可溶物种）。MBG-75 S与Saos-2成骨样细胞接触后显示出较高的生物相容性。高达1 mg/ml的浓度不会导致形态学或细胞周期的显著改变。关于对破骨细胞的影响，MBG-75 S允许RAW-264.7巨噬细胞分化成破骨细胞样细胞，但表现出降低的再吸收活性。这些结果指出，MBG-75 S不抑制破骨细胞生成，但降低破骨细胞骨吸收能力。最后，体外研究集中在先天性免疫应答上，证明MBG-75 S允许巨噬细胞增殖，而不诱导它们向M1促炎表型极化。这种体外行为表明MBG-75 S在体内条件下仅诱导所需的先天性免疫应答而没有进一步的炎性并发症。关于成骨细胞、破骨细胞和巨噬细胞的整体行为，使得该MBG成为骨移植应用于骨质疏松患者的非常有趣的候选物。
英文摘要：
A mesoporous bioactive glass (MBG) of molar composition 75SiO2-20CaO-5P2O5 (MBG-75S) has been synthetized as a potential bioceramic for bone regeneration purposes. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption studies and transmission electron microscopy (TEM) demonstrated that MBG-75Spossess a highly ordered mesoporous structure with high surface area and porosity, which would explain the high ionic exchange rate (mainly calcium and silicon soluble species) with the surrounded media. MBG-75S showed high biocompatibility in contact with Saos-2 osteoblast-like cells. Concentrations up to 1 mg/ml did not lead to significant alterations on either morphology or cell cycle. Regarding the effects on osteoclasts, MBG-75S allowed the differentiation of RAW-264.7 macrophages into osteoclast-like cells but exhibiting a decreased resorptive activity. These results point out that MBG-75S does not inhibit osteoclastogenesis but reduces the osteoclast boneresorbing capability. Finally, in vitro studies focused on the innate immune response, evidenced that MBG-75S allows the proliferation of macrophages without inducing their polarization towards the M1 pro-inflammatory phenotype. This in vitro behavior is indicative that MBG-75S would just induce the required innate immune response without further inflammatory complications under in vivo conditions. The overall behavior respect to osteoblasts, osteoclasts and macrophages, makes this MBG a very interesting candidate for bone grafting applications in osteoporotic patients.
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