利用纳米金刚石磁测量法检测脂质双层膜的无标记相变
Label-free phase change detection of lipid bilayers using nanoscale diamond magnetometry
摘要：
金刚石中的NV中心是一种质量卓越的量子传感器，可用于NMR光谱和温度测量的高灵敏度纳米级分析。在这项研究中，我们研究了纳米相变检测的脂质双层利用整体平均核自旋检测从小体积~（6 nm）$^{3}$，这是由NV中心的深度确定。纳米级NMR信号的分析证实脂质双层的厚度为6.2 nm $\pm$ 3.4 nm，质子密度为65质子/nm $^{3}$，证实在金刚石样品顶部形成脂质双层。从纳米尺度体积的相关光谱揭示了量子振荡在3.06 MHz对应的拉莫尔频率的质子在施加的磁场为71.8 mT。将相关谱的计算结果与蒙特卡罗模拟建立的二维分子扩散模型结合分子动力学模拟的结果进行了比较。当温度从26.5 $^\circ$ C变化到36.0 $^\circ$ C时，扩散常数从1.5 $\pm$ 0.25 nm $^{2}$/$μ$ s变化到3.0 $\pm$ 0.5 nm $^{2}$ / $μ$ s。我们的研究结果表明，同时观察平移扩散和温度的变化是可能的，在无标记测量使用纳米金刚石磁强计。我们的方法为细胞膜的无标记成像铺平了道路，以了解其相组成和动力学。
英文摘要：
The NV center in a diamond is a quantum sensor with exceptional quality for highly sensitive nanoscale analysis of NMR spectra and thermometry. In this study, we investigate nanoscale phase change detection of lipid bilayers utilizing ensemble-averaged nuclear spin detection from small volume ~ (6 nm)$^{3}$, which was determined by the depth of the NV center. Analysis of nanoscale NMR signal confirm thickness of lipid bilayer to be 6.2 nm $\pm$ 3.4 nm with proton density of 65 proton/nm$^{3}$ verifying formation of lipid bilayer on top of diamond sample. Correlation spectroscopy from nanoscale volume reveals quantum oscillation at 3.06 MHz corresponding to the Larmor frequency of proton at an applied magnetic field of 71.8 mT. The result of the correlation spectroscopy was compared with the 2D molecular diffusion model constructed by Monte Carlo simulation combined with results from molecular dynamics simulation. There is a change in diffusion constant from 1.5 $\pm$ 0.25 nm$^{2}$/$μ$s to 3.0 $\pm$ 0.5 nm$^{2}$/$μ$s when the temperature changes from 26.5 $^\circ$C to 36.0 $^\circ$C. Our results demonstrate that simultaneous observation of changes in translational diffusion and temperature is possible in label-free measurements using nanoscale diamond magnetometry. Our method paves the way for label-free imaging of cell membranes for understanding its phase composition and dynamics.
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