High-resolution label-free imaging of tissue morphology with confocal phase microscopy

Spotlight on nerves: Portable multispectral optoacoustic imaging of peripheral nerve vascularization and morphology

Various morphological and functional parameters of peripheral nerves and their vascular supply are indicative of pathological changes due to injury or disease. Based on recent improvements in optoacoustic image quality, we explore the ability of multispectral optoacoustic tomography, in tandem with ultrasound imaging (OPUS), to investigate the vascular environment and morphology of peripheral nerves in vivo in a pilot study on healthy volunteers. We showcase the unique ability of optoacoustic imaging to visualize the vasa nervorum by observing intraneurial vessels in healthy nerves in vivo for…

Neural network prediction of load from the morphology of trabecular bone

Bone adaptation models are often solved in the forward direction, meaning that the response of bone to a given set of loads is determined by running a bone tissue adaptation model. The model is generally solved using a numerical technique such as the finite element model. Conversely, one may be interested in the loads that have resulted in a given state of bone. This is the inverse of the former problem. Even though the inverse problem has several applications, it has not received as much attention as the forward problem, partly because solving the inverse problem is more difficult. A nonlinea…

血管流固耦合中的纤维组织建模：一种基于形态学的流程及其生物力学意义

Modeling fibrous tissue in vascular fluid-structure interaction: a morphology-based pipeline and biomechanical significance

本研究提出一套基于形态学的厚壁动脉纤维组织建模流程，含网格生成、局部基向量构建、多分支中心线识别等，验证表明其纤维定义效果良好，且证实动脉壁本构模型显著影响血管变形与应力分布，为精准血管建模提供支撑。

Pixel-reassignment in Ultrasound Imaging

We present an adaptation of the pixel-reassignment technique from confocal fluorescent microscopy to coherent ultrasound imaging. The method, Ultrasound Pixel-Reassignment (UPR), provides a resolution and signal to noise (SNR) improvement in ultrasound imaging by computationally reassigning off-focus signals acquired using traditional plane-wave compounding ultrasonography. We theoretically analyze the analogy between the optical and ultrasound implementations of pixel reassignment, and experimentally evaluate the imaging quality on tissue-mimicking acoustic phantoms. We demonstrate that UPR p…

Intraoperative robotic-assisted large-area high-speed microscopic imaging and intervention

Objective: Probe-based confocal endomicroscopy is an emerging high-magnification optical imaging technique that provides in vivo and in situ cellular-level imaging for real-time assessment of tissue pathology. Endomicroscopy could potentially be used for intraoperative surgical guidance, but it is challenging to assess a surgical site using individual microscopic images due to the limited field-of-view and difficulties associated with manually manipulating the probe. Methods: In this paper, a novel robotic device for large-area endomicroscopy imaging is proposed, demonstrating a rapid, but hig…

Reflection-mode virtual histology using photoacoustic remote sensing microscopy

Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging. These processes are labor intensive and contribute to delays in clinical feedback that can extend to two to three weeks for standard paraffin-embedded tissue preparation and interpretation. Here, we present a label-free reflection-mode imaging modality that reveals cellular-scale morphology by detecting intrinsic endogenous contrast. We accomplish this with…

光片显微镜评估癌症病理：当前观点与未来趋势

Light-sheet microscopy to assess cancer pathology: current views and future trends

本文综述了光片荧光显微镜在癌症病理分析中的当前应用，并探讨其整合入临床流程以实现完整标本高通量三维成像，从而提升诊断精度和揭示肿瘤结构形态的潜力。

Single Acquisition Label-free Histology-like Imaging with Dual Contrast Photoacoustic Remote Sensing Microscopy

Significance: Histopathological analysis of tissues is an essential tool for grading, staging, diagnosing and resecting cancers and other malignancies. Current histopathological techniques require substantial sample processing prior to staining with hematoxylin and eosin (H&E) dyes, to highlight nuclear and cellular morphology. Sample preparation and staining is resource-intensive and introduces potential for variability during sample preparation. Aim: We present a novel method for direct label-free histopathological assessment of formalin fixed paraffin embedded tissue blocks and thin tissue …

Short Review: Pathology of the image big data era using electron microscopy

The human body is mysterious, consisting of innumerable fine and complex structures and a vast amount of information. The field of modern pathology has been systematized around the findings and observations obtained from light microscopy. However, such observations in the visible light range are limited to those at the cellular level; thus, observations of cellular ultrastructures are difficult. Therefore, only vague information can be collected. Various imaging methodologies and tissue staining techniques have been created to further understand the complexity of biological tissues. Using such…

利用In-and-Out网络实现单色数字H&E染色

Single color digital H&E staining with In-and-Out Net

本文提出基于GAN的In-and-Out网络，将反射共聚焦显微图像高效转换为H&E染色图像，并通过氯化铝预处理和双通道虚拟标签训练，在无需配准的情况下实现了最先进的虚拟染色性能。

Histopathology for Mohs Micrographic Surgery with Photoacoustic Remote Sensing Microscopy

Mohs micrographic surgery (MMS) is a precise oncological technique where layers of tissue are resected and examined with intraoperative histopathology to minimize the removal of normal tissue while completely excising the cancer. To achieve intraoperative pathology, the tissue is frozen, sectioned and stained over a 20- to 60-minute period, then analyzed by the MMS surgeon. Surgery is continued one layer at a time until no cancerous cells remain, meaning MMS can take several hours to complete. Ideally, it would be desirable to circumvent or augment frozen sectioning methods and directly visual…