Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light emerging from a specimen, to extract a three-dimensional representation of the object from a single high-speed measurement. Until now, CI methods could not be applied to incoherent light, severely limiting their ability to image the most powerful biological probes available - fluorescent molecules - with sufficient speed and volume to observe important processes, such as neural processing in live specimens. We introduce a new imaging technique that transfers the spatial propagation phase of coherent illumination light to incoherent fluorescent light emission. The transfer of propagation phase allows CI techniques to be applied to fluorescent light imaging, and leads to large increases in imaging speed and depth of field. With this advance, biological imaging of fluorescent molecules is significantly expanded.
Label-Free Microrefractometry of Interfacial Processes Using Fluorescent Smart Coverslips
Institute of Nanotechnology and Advanced Materials (BINA)（纳米技术与先进材料研究所（BINA））；Department of Chemistry, Bar-Ilan University（巴伊兰大学化学系）；Université Paris Cité, CNRS, Saint-Pères Paris Institute for the Neurosciences（巴黎西岱大学、法国国家科学研究中心、圣佩雷斯巴黎神经科学研究所）
Full-Field Metasurface Characterization with Polarization Sensitive Coherent Modulation Imaging
State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, Zhejiang University（浙江大学光电科学与工程学院极端光子学与仪器全国重点实验室）；Department of Electrical and Electronic Engineering, The University of Hong Kong（香港大学电子电气工程系）；College of Optical and Electronic Engineering, China Jiliang University（中国计量大学光电工程学院）；ZJU-Hangzhou Global Scientific and Technological Innovation Center（浙大杭州全球科创中心）
Université Libre de Bruxelles（布鲁塞尔自由大学）；University of Lille, CNRS, UMR 8523 PhLAM（里尔大学，法国国家科学研究中心，UMR 8523 光子与激光联合实验室）；California Institute of Technology（加州理工学院）
