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arXiv 2406.03192physics.optics
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高灵敏 AuNCs@GSH/Ch-PtNPs 金属纳米探针用于饮料中抗坏血酸的荧光与比色双模式检测

Highly sensitive AuNCs@GSH/Ch-PtNPs metal nanoprobes for fluorescent and colorimetric dual-mode detection of ascorbic acid in drink

Wei Zheng

AI总结:

本文提出基于 AuNCs@GSH/Ch-PtNPs 的荧光与比色双模式金属纳米探针,用于检测饮料中的抗坏血酸,克服了单模式荧光检测的背景干扰和低浓度灵敏度低等局限,实现了高灵敏度、快速且准确的定量分析。

摘要:

荧光检测是一种常用的分析方法,具有响应快、选择性好和破坏性低的优点。然而,荧光检测作为一种单模式检测方法存在一些局限性,例如影响荧光信号准确性的背景干扰、缺乏检测结果的可视化,以及对低浓度样品检测的灵敏度低。为了克服荧光单模式检测的缺点,我们采用荧光与比色双模式方法检测抗坏血酸。在探针系统中通过荧光和比色法对 AA 进行双模式检测,增强了检测的特异性和准确性。这种双峰检测方法解决了待测分析物低浓度范围内检测灵敏度低的问题,分别在较低(0-50 μM)和较高(50-350 μM)浓度范围内呈线性,并具有较低的检测限(0.034 μM)。这种基于谷胱甘肽的金簇检测法具有简便、快速和准确的特点,为抗坏血酸的定量分析提供了新途径。此外,该方法在饮料中测定 AA 时得到了验证,具有灵敏度高和响应时间快的优点。

英文摘要:

Fluorescence detection is a commonly used analytical method with the advantages of fast response, good selectivity and low destructiveness. However, fluorescence detection, a single-mode detection method, has some limitations, such as background interference that affects the accuracy of the fluorescence signal, lack of visualization of the detection results, and low sensitivity for detecting low-concentration samples. In order to overcome the shortcomings of fluorescence single-mode detection, we used the dual-mode method of fluorescence and colorimetry to detect ascorbic acid. The dual-mode detection of AA by fluorescence and colorimetry in the probe system enhances the specificity and accuracy of the detection. This bimodal detection method solved the problem of low detection sensitivity in the low concentration range of the analytes to be tested, and was linear in the lower (0-50 μM) and higher (50-350 μM) concentration ranges, respectively, and had a lower detection limit (0.034 μM). This glutathione-based gold cluster assay is characterized by simplicity, rapidity and accuracy, and provides a new way for the quantitative analysis of ascorbic acid. In addition, the method was validated during the determination of AA in beverages, which has the advantages of high sensitivity and fast response time.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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