生长速率的波动决定了E.杆菌细胞
Fluctuations in growth rates determine the generation time and size distributions of E. coli cells
摘要：
同基因大肠杆菌在恒定的环境中呈指数生长，其生长速率、分裂大小和世代时间都有很大的差异。目前还不清楚这些看似随机的细胞周期如何与在世代时间短于复制基因组时间的条件下所需的精确调控相协调。在这里，我们使用单分子显微镜来映射复制机制的位置到单个细胞的分裂周期。我们发现，细胞与细胞的生长速率的变化是足够的，以解释相应的细胞大小和分裂时间的变化，假设一个简单的机械模型。在该模型中，染色体复制的起始是在每个起始区域的固定体积下触发的，并且与每个起始事件相关联的是随后在生长速率依赖性时间的分裂事件。结果表明，E.大肠杆菌除了启动DNA复制所需的适当体积外，没有其他调节。
英文摘要：
Isogenic Escherichia coli growing exponentially in a constant environment display large variation in growth-rates, division-sizes and generation-times. It is unclear how these seemingly random cell cycles can be reconciled with the precise regulation required under conditions where the generation time is shorter than the time to replicate the genome. Here we use single molecule microscopy to map the location of the replication machinery to the division cycle of individual cells. We find that the cell-to-cell variation in growth rate is sufficient to explain the corresponding variation in cell size and division timing assuming a simple mechanistic model. In the model, initiation of chromosome replication is triggered at a fixed volume per origin region, and associated with each initiation event is a division event at a growth rate dependent time later. The result implies that cell division in E. coli has no other regulation beyond what is needed to initiate DNA replication at the right volume.
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