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arXiv 1209.1744physics.opticsq-bio.QM
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由光场中的散焦信息进行相位成像

Phase imaging from defocus information in a light field

Quintin Tyrell Davis

摘要:

光学显微镜无疑是生命科学研究的重要组成部分,但生物学中许多感兴趣的物体都是透明的。化学或免疫染料,其通常是有毒的,荧光转基因,其需要用于转化外源DNA的协议,以及相位对比,其不可避免地将相位信息与振幅相结合,所有这些都用于增加透明物体的对比度。非均匀折射率和/或厚度的透明物体是相位物体,并且可以从物体上方和下方的散焦信息中提取相位。光学显微镜将认识到这一点,因为物体的对比度可以随着载物台围绕物体的焦平面移动而变化。该相位信息可以从一组散焦图像中定量地检索,但这具有降低时间分辨率的缺点,因为显微镜载物台必须在图像捕获之间移动。通过采用针孔或微透镜阵列形式的附加光学器件,所有光线的方向和位置都可以记录在光场中,这是一种4D功能。通过记录光场,可以研究多个焦平面,从而允许实时捕获散焦信息。本文讨论和研究了利用光场的散焦信息来获得光强输运方程,并利用快速傅里叶变换方法求解该方程的相位。

英文摘要:

Optical microscopy is without a doubt an essential component of life science research, but many objects of interest in biology are transparent. Chemical or immunological dyes, which can often be toxic, fluorescent transgenes, which require a protocol for transformation of exogenous DNA, and phase contrast, which inextricably combines phase information with amplitude, all serve to increase the contrast of transparent objects. Transparent objects of non-uniform refractive index and/or thickness are phase objects and phase can be extracted from defocus information above and below the object. The light microscopist will recognize this in that object contrast can vary as the stage is moved around an object's focal plane. This phase information can be quantitatively retrieved from a set of defocused images, but this has the disadvantage of decreasing temporal resolution, as the microscope stage has to be moved between image captures. By incorporating additional optics in the form of an array of pinholes or microlenses, the direction and position of all the rays can be recorded in a light field, a 4D function. By recording the light field, multiple planes of focus can be investigated, allowing for the capture of defocus information in real time. Here I discuss and investigate the use of the defocus information in a light field to obtain the transport of intensity equation, and use the fast Fourier transform method to solve this equation for phase.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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