用FTIR光谱法研究UVB辐射对细胞的影响
UVB radiation induced effects on cells studied by FTIR spectroscopy
摘要：
本文对UVB辐射（剂量为310 mJ/cm ^2）对肿瘤细胞系（淋巴T细胞系Jurkat）的影响作了初步分析。在本研究中，我们使用了两种技术：傅里叶变换红外光谱（FTIR）和流式细胞术。FTIR光谱学具有提供一些主要化合物（脂质、蛋白质和核酸）的振动模式的识别而不侵入生物材料的潜力。第二种技术使我们能够进行细胞毒性的测量，并评估细胞凋亡的百分比。我们已经研究了通过UVB辐射在相同细胞系中诱导凋亡过程;特别地，我们寻找FTIR光谱和流式细胞术数据之间的对应性和相关性，发现了三种高度可能的凋亡光谱标记物（Pozzi等人，Radiat Res 168：698-705，2007）。在目前的工作中，结果表明，显着的变化，在波数蛋白质和核酸区域的吸光度和光谱图案后的处理。
英文摘要：
We have made a preliminary analysis of the results about the eVects on tumoral cell line (lymphoid T cell line Jurkat) induced by UVB radiation (dose of 310 mJ/cm^2) with and without a vegetable mixture. In the present study, we have used two techniques: Fourier transform infrared spectroscopy (FTIR) and flow cytometry. FTIR spectroscopy has the potential to provide the identiWcation of the vibrational modes of some of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The second technique has allowed us to perform measurements of cytotoxicity and to assess the percentage of apoptosis. We already studied the induction of apoptotic process in the same cell line by UVB radiation; in particular, we looked for correspondences and correlations between FTIR spetroscopy and flow cytometry data finding three highly probable spectroscopic markers of apoptosis (Pozzi et al. in Radiat Res 168:698-705, 2007). In the present work, the results have shown significant changes in the absorbance and spectral pattern in the wavenumber protein and nucleic acids regions after the treatments.
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