ATR-FTIR光谱检测在亚细胞毒性/遗传毒性浓度下MCF-7细胞中有机锡（IV）羧酸盐诱导的变化
ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations
摘要：
金属络合物如有机锡（IV）化合物的环境影响日益受到关注。采用碱性单细胞凝胶电泳（彗星）试验测量DNA单链断裂（SSB），并采用胞分裂阻滞微核（CBMN）试验确定微核形成，从而测量有机锡（IV）化合物（0.01 μ g/ml、0.1 μ g/ml或1.0 μ g/ml）的遗传毒性效应。生物化学细胞的签名也确定使用衰减全反射傅里叶变换红外（ATR-FTIR）光谱。在彗星试验中，有机锡（IV）羧酸盐诱导显着升高的DNA SSB水平。还观察到微核形成活性升高。在使用ATR-FTIR光谱进行询问后，在生物分子范围内（900 cm-1 - 1800 cm-1）的红外光谱来自有机物。
英文摘要：
The environmental impact of metal complexes such as organotin(IV) compounds is of increasing concern. Genotoxic effects of organotin(IV) compounds (0.01 microg/ml, 0.1 microg/ml or 1.0 microg/ml) were measured using the alkaline single-cell gel electrophoresis (comet) assay to measure DNA single-strand breaks (SSBs) and the cytokinesis-block micronucleus (CBMN) assay to determine micronucleus formation. Biochemical-cell signatures were also ascertained using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. In the comet assay, organotin(IV) carboxylates induced significantly-elevated levels of DNA SSBs. Elevated micronucleus-forming activities were also observed. Following interrogation using ATR-FTIR spectroscopy, infrared spectra in the biomolecular range (900 cm-1 - 1800 cm-1) derived from orga...
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