UVB辐射诱导Jurkat细胞凋亡的协同傅里叶变换红外光谱-流式细胞术研究
UVB-Radiation-Induced Apoptosis in Jurkat Cells: A Coordinated Fourier Transform Infrared Spectroscopy-Flow Cytometry Study
摘要：
我们研究了剂量为310 mJ/cm ^2的UVB辐射（波长290-320 nm）对Jurkat细胞凋亡的诱导作用。我们将傅里叶变换红外（FTIR）光谱与流式细胞术相结合，以确定这两种技术的结合是否可以提供有关细胞修饰的新的和改进的信息。为此，我们寻找光谱和流式细胞术数据之间的对应关系和相关性，并发现了三个高度可能的凋亡光谱标记。酰胺I β折叠组分的波数移动行为和1083 cm^-1条带的面积再现了早期凋亡细胞群体的行为，且相关性很高，而酰胺I区域的行为显示出与早期和晚期凋亡细胞群体的高度相关性。
英文摘要：
We studied the induction of apoptosis in Jurkat cells by UVB radiation (wavelength 290-320 nm) at a dose of 310 mJ/cm^2. We combined Fourier transform infrared (FTIR) spectroscopy with flow cytometry to determine whether the combination of both techniques could provide new and improved information about cell modifications. To do this, we looked for correspondences and correlations between spectroscopy and flow cytometry data and found three highly probable spectroscopic markers of apoptosis. The behavior of the wave number shift of both the Amide I beta-sheet component and the area of the 1083 cm^-1 band reproduced, with a high correlation, the behavior of the early apoptotic cell population, while the behavior of the Amide I area showed a high correlation with the early plus late apoptotic cell population.
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