半导体量子点与不闪烁钻石奈米粒子光致发光特性之比较。金刚石纳米颗粒在活细胞中扩散的观察
Comparison of the photoluminescence properties of semiconductor quantum dots and non-blinking diamond nanoparticles. Observation of the diffusion of diamond nanoparticles in living cells
摘要：
在单分子水平的光致发光的长期观察，直到最近非常困难，由于光漂白的有机？荧光团分子。虽然无机半导体纳米晶体可以克服这一困难，显示出非常低的光漂白产率，但它们遭受光闪烁。最近引入了一种新的标记，依赖于含有光致发光色心的金刚石纳米颗粒。在这项工作中，我们比较了单量子点（QD）的光致发光的纳米金刚石含有一个单一的颜色中心。与其他标记相反，光致发光纳米金刚石具有完美的光稳定性，并且没有光闪烁。在它们的激发饱和时，纳米金刚石的光致发光强度仅比量子点的光致发光强度小三倍。此外，纳米金刚石的明亮和稳定的光致发光允许宽场观察单个纳米颗粒的运动。我们证明了记录这种单个粒子在培养细胞中的轨迹的可能性。
英文摘要：
Long-term observations of photoluminescence at the single-molecule level were until recently very diffcult, due to the photobleaching of organic ?uorophore molecules. Although inorganic semiconductor nanocrystals can overcome this diffculty showing very low photobleaching yield, they suffer from photoblinking. A new marker has been recently introduced, relying on diamond nanoparticles containing photoluminescent color centers. In this work we compare the photoluminescence of single quantum dots (QDs) to the one of nanodiamonds containing a single-color center. Contrary to other markers, photoluminescent nanodiamonds present a perfect photostability and no photoblinking. At saturation of their excitation, nanodiamonds photoluminescence intensity is only three times smaller than the one of QDs. Moreover, the bright and stable photoluminescence of nanodiamonds allows wide field observations of single nanoparticles motion. We demonstrate the possibility of recording the tra jectory of such single particle in culture cells.
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