分子电穿孔与寡聚腺苷酸的转导
Molecular Electroporation and the Transduction of Oligoarginines
摘要：
某些短的聚阳离子，如TAT和聚精氨酸，通过一种称为转导的未知机制以及通过内吞作用和巨胞饮作用快速穿过哺乳动物细胞的质膜。这些细胞穿透肽（CPP）融合到生物活性肽时，有望成为医学上有用的。我提供了一个简单的模型，其中一个或多个CPP和内部小叶的磷脂酰丝氨酸形成一种电容器，电压超过180 mV，高到足以产生分子电孔。该模型与40- 60个氨基酸的货物肽的经验上限一致，并且与关于聚精氨酸荧光团转导到小鼠C2 C12成肌细胞中如何取决于CPP中精氨酸的数量和CPP浓度的实验数据一致。该模型做出了三个可检验的预测。
英文摘要：
Certain short polycations, such as TAT and polyarginine, rapidly pass through the plasma membranes of mammalian cells by an unknown mechanism called transduction as well as by endocytosis and macropinocytosis. These cell-penetrating peptides (CPPs) promise to be medically useful when fused to biologically active peptides. I offer a simple model in which one or more CPPs and the phosphatidylserines of the inner leaflet form a kind of capacitor with a voltage in excess of 180 mV, high enough to create a molecular electropore. The model is consistent with an empirical upper limit on the cargo peptide of 40--60 amino acids and with experimental data on how the transduction of a polyarginine-fluorophore into mouse C2C12 myoblasts depends on the number of arginines in the CPP and on the CPP concentration. The model makes three testable predictions.
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