利用贝叶斯反演应力显微镜测量组织应力
Tissue stress measurements with Bayesian Inversion Stress Microscopy
AI总结：
本研究提出了一种无需假设细胞流变学的贝叶斯反演应力显微镜技术，用于测量复杂异质组织中的应力，适用于多种实验条件和细胞类型。
摘要：
生物组织内的细胞始终受到动态机械力的作用。测量组织内部应力已被证明对理解机械力在基本生物学过程中的作用至关重要，如形态发生、集体迁移、细胞分裂或细胞消除和死亡。此前，我们介绍了贝叶斯反演应力显微镜（BISM），该方法依赖于在体外测量细胞产生的牵引力，并已证明特别适用于测量受限细胞单层中的绝对应力。我们进一步展示了BISM在不同实验设置中的适用性和鲁棒性，从任意形状的受限组织到由不同细胞类型组成的单层。重要的是，BISM不需要对细胞流变学做出假设。因此，只要这些细胞可以生长在平底基质上，它就可以应用于由不同细胞类型组成的复杂异质组织。最后，我们使用一个相干的实验设置将BISM与其他常见应力测量技术进行比较，随后讨论其局限性和进一步的前景。
英文摘要：
Cells within biological tissue are constantly subjected to dynamic mechanical forces. Measuring the internal stress of tissues has proven crucial for our understanding of the role of mechanical forces in fundamental biological processes like morphogenesis, collective migration, cell division or cell elimination and death. Previously, we have introduced Bayesian Inversion Stress Microscopy (BISM), which is relying on measuring cell-generated traction forces in vitro and has proven particularly useful to measure absolute stresses in confined cell monolayers. We further demonstrate the applicability and robustness of BISM across various experimental settings with different boundary conditions, ranging from confined tissues of arbitrary shape to monolayers composed of different cell types. Importantly, BISM does not require assumptions on cell rheology. Therefore, it can be applied to complex heterogeneous tissues consisting of different cell types, as long as they can be grown on a flat substrate. Finally, we compare BISM to other common stress measurement techniques using a coherent experimental setup, followed by a discussion on its limitations and further perspectives.
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