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arXiv 2502.13664physics.optics
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利用回音壁模式微激光器进行 DNA 传感

DNA Sensing with Whispering Gallery Mode Microlasers

Soraya Caixeiro, Robert Dörrenhaus, Anna Popczyk, Marcel Schubert, Stephanie Kath-Schorr, Malte C. Gather

AI总结:

本文提出 DNA 功能化 WGM 微激光传感平台,利用光谱位移实时检测 DNA 杂交与结构变化,并通过金纳米颗粒和发夹结构提升灵敏度、简化标记及支持控释应用。

摘要:

核酸传感通过实现对 DNA 和 RNA 相互作用的精确识别,对于推进诊断、治疗监测和分子生物学研究至关重要。本文提出一种基于 DNA 功能化回音壁模式(WGM)微激光器的创新传感平台。通过将激光发射的光谱位移与折射率变化相关联,我们展示了对 DNA 杂交和结构变化的实时检测。在 DNA 链上加入金纳米颗粒可显著提高灵敏度,并且仅标记传感链或发夹链即可免除对目标链进行二次标记的需要。我们进一步表明,离子强度会影响 DNA 的紧密程度,并引入一种基于发夹的系统,作为兼具传感和控释机制的双重用途装置,具有潜在的药物递送应用。这种多功能的基于 WGM 的平台为序列特异性核酸传感、多重检测以及诊断和细胞研究中的体内应用提供了前景。

英文摘要:

Nucleic acid sensing is crucial for advancing diagnostics, therapeutic monitoring and molecu-lar biology research, by enabling the precise identification of DNA and RNA interactions. Here, we present an innovative sensing platform based on DNA-functionalized whispering gallery mode (WGM) microlasers. By correlating spectral shifts in laser emission to changes in refractive index, we demonstrate real-time detection of DNA hybridization and structural changes. The addition of gold nanoparticles to the DNA strands significantly enhances sensi-tivity, and labeling exclusively the sensing strand or a hairpin strand eliminates the need for secondary labeling of the target strand. We further show that ionic strength influences DNA compactness, and we introduce a hairpin-based system as a dual-purpose sensor and con-trolled release mechanism for potential drug delivery. This versatile WGM-based platform of-fers promise for sequence-specific nucleic acid sensing, multiplexed detection, and in vivo ap-plications in diagnostics and cellular research.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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