arXiv 2409.014852024-09-02physics.opticsphysics.bio-ph

回音壁模式微激光器的光学操控用于精准控制的细胞递送

Optical Manipulation of Whispering Gallery Mode Microlasers for Precision Controlled Cellular Delivery

Soraya Caixeiro, Paloma Rodríguez-Sevilla, Kishan Dholakia, Malte C Gather

备注：: 16 pages, 5 figures

阅读 PDF

arXiv 页面 ↗HTML 原文 ↗

AI总结：

本文提出利用光镊实现回音壁模式微激光器的受控、非侵入性细胞递送，并结合折射率传感研究细胞摄取动力学，发现光谱变化可反映微激光器周围局部折射率不均匀性，从而扩展了生物集成微激光器的应用工具集。

摘要：

回音壁模式微激光器以其高品质因子、特征发射光谱以及对局部折射率变化的敏感性而闻名。这种敏感性与多种细胞类型内化这些微激光器的能力相结合，为高级生物学研究（例如单细胞追踪和细胞内传感）提供了独特的机会。尽管取得了许多进展，但实现激光器向细胞的精准递送仍然具有挑战性，传统方法（如显微注射）通常还会导致细胞损伤。在此，我们表明光镊是微激光器操控的一种有前景的解决方案，因此可用于对其目标细胞进行受控且非侵入性的递送。通过将光镊与基于微激光器的折射率传感相结合，我们研究了细胞摄取微激光器的动力学过程。我们还发现，在某些情况下，我们的微激光器发射光谱中的峰会发生展宽、分裂和移动，我们将其归因于微激光器周围局部折射率的不均匀性。这表明光镊能够进一步扩展生物集成回音壁模式微激光器所提供的独特工具集。

英文摘要：

Whispering gallery mode microlasers are known for their high Q-factors, characteristic emission spectra and sensitivity to local refractive index changes. This sensitivity combined with the ability of various cell types to internalise these microlasers provide unique opportunities for advanced biological studies, e.g. in single-cell tracking and intracellular sensing. Despite many advancements, achieving precise delivery of lasers to cells remains challenging, with traditional methods, such as microinjection, often also resulting in cellular damage. Here, we show that optical trapping is a promising solution for microlaser manipulation and therefore for their controlled and non-invasive delivery to target cells. By integrating optical trapping with microlaser-based refractive index sensing, we study the dynamics of microlaser-uptake by cells. We also find that in some cases the peaks in the emission spectra of our microlasers broaden, split, and shift, and we assign this to local inhomogeneities in refractive index surrounding the microlaser. This shows how optical trapping can further expand the unique toolkit offered by biointegrated whispering gallery mode microlasers.

机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。

AI总结：

摘要：

英文摘要：

相关论文