近红外超构透镜赋能的双模高分辨率与大视场显微镜
Near-infrared metalens empowered dual-mode high resolution and large FOV microscope
AI总结:
本文提出一种基于近红外超构透镜的紧凑型双模显微镜,通过偏振控制实现螺旋相位对比与明场成像切换,兼具亚波长分辨率、大放大率与大视场,适用于生物成像及工业检测。
摘要:
螺旋相位对比显微镜因其各向同性的边缘增强效应,能清晰区分低对比度物体(即生物样品)的形态信息,而明场显微镜则能对振幅物体的整体形态进行成像。然而,基于4f滤波配置的传统螺旋相位对比显微镜,其成像分辨率、放大率及视场受限于系统复杂性。在此,我们利用工程化超构透镜,报道了工作在近红外波段的紧凑型双模显微镜,该显微镜可通过偏振控制,在螺旋相位对比成像与明场成像之间切换。该超构透镜将高分辨率物镜和偏振控制相位滤波器集成到单层纳米鳍阵列中。我们演示了两个无限远校正显微镜系统,实现了亚波长分辨率(0.7倍波长)、大放大率(58X)和大视场(600um×800um)。使用未染色的洋葱表皮进行成像,展示了该显微镜对生物样品的双模成像能力。最后,演示了单透镜双模显微镜系统,展示了其在工业标准中的边缘检测应用。我们的结果可为生物成像、工业机器视觉及半导体检测的应用开辟新机遇。
英文摘要:
The spiral phase contrast microscope can clearly distinguish the morphological information of the low contrast objects (i.e., biological samples) because of the isotropic edge-enhancement effect, while the bright field microscope can image the overall morphology of amplitude objects. However, the imaging resolution, magnification, and field of view of conventional spiral phase contrast microscopes based on 4f filtering configuration are limited by the system's complexity. Here, we reported compact dual-mode microscopes working at near-infrared using the engineered metalens which can be tuned between the spiral phase contrast imaging and bright field imaging by polarization control. The metalens combines the high-resolution objective lens and polarization-controlled phase filter into a single-layer nanofins array. We demonstrated two infinity-corrected microscope systems to achieve subwavelength resolution (0.7 times of wavelength), large magnification (58X), and large field of view (600um times 800um). Unstained onion epidermal is imaged by the microscope to show the dual-mode imaging ability for the biological sample. Finally, a singlet dual-mode microscope system is demonstrated to show the edge-detection application for industrial standards. Our results could open new opportunities in applications of biological imaging, industrial machine vision, and semiconductor inspection.
机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。