A standard treatment protocol for breast cancer entails administering neoadjuvant therapy followed by surgical removal of the tumor and surrounding tissue. Pathologists typically rely on cabinet X-ray radiographs, known as Faxitron, to examine the excised breast tissue and diagnose the extent of residual disease. However, accurately determining the location, size, and focality of residual cancer can be challenging, and incorrect assessments can lead to clinical consequences. The utilization of automated methods can improve the histopathology process, allowing pathologists to choose regions for sampling more effectively and precisely. Despite the recognized necessity, there are currently no such methods available. Training such automated detection models require accurate ground truth labels on ex-vivo radiology images, which can be acquired through registering Faxitron and histopathology images and mapping the extent of cancer from histopathology to x-ray images. This study introduces a deep learning-based image registration approach trained on mono-modal synthetic image pairs. The models were trained using data from 50 women who received neoadjuvant chemotherapy and underwent surgery. The results demonstrate that our method is faster and yields significantly lower average landmark error ($2.1\pm1.96$ mm) over the state-of-the-art iterative ($4.43\pm4.1$ mm) and deep learning ($4.02\pm3.15$ mm) approaches. Improved performance of our approach in integrating radiology and pathology information facilitates generating large datasets, which allows training models for more accurate breast cancer detection.
We propose a virtual staining methodology based on Generative Adversarial Networks to map histopathology images of breast cancer tissue from H&E stain to PHH3 and vice versa. We use the resulting synthetic images to build Convolutional Neural Networks (CNN) for automatic detection of mitotic figures, a strong prognostic biomarker used in routine breast cancer diagnosis and grading. We propose several scenarios, in which CNN trained with synthetically generated histopathology images perform on par with or even better than the same baseline model trained with real images. We discuss the potentia…
Top Data Science Ltd.（顶级数据科学有限公司）；University of Helsinki（赫尔辛基大学）；Skåne University Hospital（斯科讷大学医院）；Helsinki University Hospital（赫尔辛基大学医院）
Deep neural networks have introduced significant advancements in the field of machine learning-based analysis of digital pathology images including prostate tissue images. With the help of transfer learning, classification and segmentation performance of neural network models have been further increased. However, due to the absence of large, extensively annotated, publicly available prostate histopathology datasets, several previous studies employ datasets from well-studied computer vision tasks such as ImageNet dataset. In this work, we propose a transfer learning scheme from breast histopath…
Jalpaiguri Government Engineering College（贾尔派古里政府工程学院）
Invasive ductal carcinoma (IDC), which is also sometimes known as the infiltrating ductal carcinoma, is the most regular form of breast cancer. It accounts for about 80% of all breast cancers. According to the American Cancer Society, more than 180,000 women in the United States are diagnosed with invasive breast cancer each year. The survival rate associated with this form of cancer is about 77% to 93% depending on the stage at which they are being diagnosed. The invasiveness and the frequency of the occurrence of these disease makes it one of the difficult cancers to be diagnosed. Our propos…
University of Toronto（多伦多大学）；National Cancer Research Centre（国家癌症研究中心）；Institute for Oncology and Radiology（肿瘤与放射学研究所）
Accurate diagnosis of breast cancer in histopathology images is challenging due to the heterogeneity of cancer cell growth as well as of a variety of benign breast tissue proliferative lesions. In this paper, we propose a practical and self-interpretable invasive cancer diagnosis solution. With minimum annotation information, the proposed method mines contrast patterns between normal and malignant images in unsupervised manner and generates a probability map of abnormalities to verify its reasoning. Particularly, a fully convolutional autoencoder is used to learn the dominant structural patter…
Northeastern University（东北大学）；Stevens Institute of Technology（史蒂文斯理工学院）；Cancer Hospital of China Medical University（中国医科大学附属肿瘤医院）；Chengdu University of Information Technology（成都信息工程大学）
Breast cancer is one of the most common and deadliest cancers among women. Since histopathological images contain sufficient phenotypic information, they play an indispensable role in the diagnosis and treatment of breast cancers. To improve the accuracy and objectivity of Breast Histopathological Image Analysis (BHIA), Artificial Neural Network (ANN) approaches are widely used in the segmentation and classification tasks of breast histopathological images. In this review, we present a comprehensive overview of the BHIA techniques based on ANNs. First of all, we categorize the BHIA systems int…
Breast cancer has the highest mortality among cancers in women. Computer-aided pathology to analyze microscopic histopathology images for diagnosis with an increasing number of breast cancer patients can bring the cost and delays of diagnosis down. Deep learning in histopathology has attracted attention over the last decade of achieving state-of-the-art performance in classification and localization tasks. The convolutional neural network, a deep learning framework, provides remarkable results in tissue images analysis, but lacks in providing interpretation and reasoning behind the decisions. …
Center for Wireless Networks & Applications (WNA), Amrita Vishwa Vidyapeetham（阿姆里塔·维什瓦·维迪亚皮塔姆大学无线网络与应用中心）；Valeo（法雷奥）；Department of Computer Science and Engineering, Amrita Vishwa Vidyapeetham（阿姆里塔·维什瓦·维迪亚皮塔姆大学计算机科学与工程系）；School of Computing and Electrical Engineering, IIT Mandi（印度理工学院曼迪分校计算与电气工程学院）
Computer assisted diagnosis in digital pathology is becoming ubiquitous as it can provide more efficient and objective healthcare diagnostics. Recent advances have shown that the convolutional Neural Network (CNN) architectures, a well-established deep learning paradigm, can be used to design a Computer Aided Diagnostic (CAD) System for breast cancer detection. However, the challenges due to stain variability and the effect of stain normalization with such deep learning frameworks are yet to be well explored. Moreover, performance analysis with arguably more efficient network models, which may…
Radboud University Medical Center（拉德堡德大学医学中心）；Beth Israel Deaconess Medical Center（贝斯以色列女执事医疗中心）；Harvard Medical School（哈佛医学院）；National Cancer Institute（美国国家癌症研究所）；The Harker School（哈克学校）；Mayo Clinic（梅奥诊所）
Diagnosis of breast carcinomas has so far been limited to the morphological interpretation of epithelial cells and the assessment of epithelial tissue architecture. Consequently, most of the automated systems have focused on characterizing the epithelial regions of the breast to detect cancer. In this paper, we propose a system for classification of hematoxylin and eosin (H&E) stained breast specimens based on convolutional neural networks that primarily targets the assessment of tumor-associated stroma to diagnose breast cancer patients. We evaluate the performance of our proposed system usin…
A Chain-of-thought Reasoning Breast Ultrasound Dataset Covering All Histopathology Categories
Peking University（北京大学）；Peking Union Medical College Hospital（北京大学人民医院）；Peking University Cancer Hospital & Institute（北京大学肿瘤医院）；National Cancer Center / National Clinical Research Center for Cancer / Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College（中国医学科学院国家癌症中心/国家癌症临床科研中心/癌症医院）；Shenzhen Maternity & Child Health Care Hospital（深圳妇幼保健医院）；Xi’an Jiaotong University（西安交通大学）；Yizhun Medical AI Co., Ltd（亿众医疗人工智能有限公司）
We present a unified framework to predict tumor proliferation scores from breast histopathology whole slide images. Our system offers a fully automated solution to predicting both a molecular data-based, and a mitosis counting-based tumor proliferation score. The framework integrates three modules, each fine-tuned to maximize the overall performance: An image processing component for handling whole slide images, a deep learning based mitosis detection network, and a proliferation scores prediction module. We have achieved 0.567 quadratic weighted Cohen's kappa in mitosis counting-based score p…
University of Warwick（华威大学）；Tissue Image Analytics (TIA) Centre, University of Warwick（华威大学组织图像分析中心）
The problem of recognizing various types of tissues present in multi-gigapixel histology images is an important fundamental pre-requisite for downstream analysis of the tumor microenvironment in a bottom-up analysis paradigm for computational pathology. In this paper, we propose a deep dictionary learning approach to solve the problem of tissue phenotyping in histology images. We propose deep Multi-Resolution Dictionary Learning (deepMRDL) in order to benefit from deep texture descriptors at multiple different spatial resolutions. We show the efficacy of the proposed approach through extensive…
University Joseph Fourier（约瑟夫·傅里叶大学）；IPAL, CNRS UMI 2955（IPAL，法国国家科学研究中心UMI 2955）；CoSMo Software（CoSMo软件公司）；Temasys Communications（Temasys通信公司）；University Pierre and Marie Curie（皮埃尔和玛丽·居里大学）
Accurate detection of mitosis plays a critical role in breast cancer histopathology. Manual detection and counting of mitosis is tedious and subject to considerable inter- and intra-reader variations. Multispectral imaging is a recent medical imaging technology, proven successful in increasing the segmentation accuracy in other fields. This study aims at improving the accuracy of mitosis detection by developing a specific solution using multispectral and multifocal imaging of breast cancer histopathological data. We propose to enable clinical routine-compliant quality of mitosis discrimination…
Automated classification of histopathological whole-slide images (WSI) of breast tissue requires analysis at very high resolutions with a large contextual area. In this paper, we present context-aware stacked convolutional neural networks (CNN) for classification of breast WSIs into normal/benign, ductal carcinoma in situ (DCIS), and invasive ductal carcinoma (IDC). We first train a CNN using high pixel resolution patches to capture cellular level information. The feature responses generated by this model are then fed as input to a second CNN, stacked on top of the first. Training of this stac…
Studies estimate that there will be 266,120 new cases of invasive breast cancer and 40,920 breast cancer induced deaths in the year of 2018 alone. Despite the pervasiveness of this affliction, the current process to obtain an accurate breast cancer prognosis is tedious and time consuming, requiring a trained pathologist to manually examine histopathological images in order to identify the features that characterize various cancer severity levels. We propose MITOS-RCNN: a novel region based convolutional neural network (RCNN) geared for small object detection to accurately grade one of the thre…
Karolinska Institutet（卡罗林斯卡学院）；Tampere University（坦佩雷大学）；Lund University（隆德大学）；University of Turku（图尔库大学）；Karolinska University Laboratory（卡罗林斯卡大学实验室）
Molecular phenotyping is central in cancer precision medicine, but remains costly and standard methods only provide a tumour average profile. Microscopic morphological patterns observable in histopathology sections from tumours are determined by the underlying molecular phenotype and associated with clinical factors. The relationship between morphology and molecular phenotype has a potential to be exploited for prediction of the molecular phenotype from the morphology visible in histopathology images. We report the first transcriptome-wide Expression-MOrphology (EMO) analysis in breast cancer,…
Northeastern University（东北大学）；China Medical University（中国医科大学）；University of Luebeck（吕贝克大学）
Background: Breast cancer has the highest prevalence in women globally. The classification and diagnosis of breast cancer and its histopathological images have always been a hot spot of clinical concern. In Computer-Aided Diagnosis (CAD), traditional classification models mostly use a single network to extract features, which has significant limitations. On the other hand, many networks are trained and optimized on patient-level datasets, ignoring the application of lower-level data labels. Method: This paper proposes a deep ensemble model based on image-level labels for the binary classificat…
Deep Learning-based Prediction of Breast Cancer Tumor and Immune Phenotypes from Histopathology
Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School（哈佛医学院麻省总医院阿蒂努拉·A·马蒂诺斯生物医学成像中心）；Faculty of Engineering, University of Porto（波尔图大学工程学院）；Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)（系统与计算机工程、技术与科学研究院）
This work presents a novel self-supervised pre-training method to learn efficient representations without labels on histopathology medical images utilizing magnification factors. Other state-of-theart works mainly focus on fully supervised learning approaches that rely heavily on human annotations. However, the scarcity of labeled and unlabeled data is a long-standing challenge in histopathology. Currently, representation learning without labels remains unexplored for the histopathology domain. The proposed method, Magnification Prior Contrastive Similarity (MPCS), enables self-supervised lear…
Despite the promise of Convolutional neural network (CNN) based classification models for histopathological images, it is infeasible to quantify its uncertainties. Moreover, CNNs may suffer from overfitting when the data is biased. We show that Bayesian-CNN can overcome these limitations by regularizing automatically and by quantifying the uncertainty. We have developed a novel technique to utilize the uncertainties provided by the Bayesian-CNN that significantly improves the performance on a large fraction of the test data (about 6% improvement in accuracy on 77% of test data). Further, we pr…
GRAPHITE: Graph-Based Interpretable Tissue Examination for Enhanced Explainability in Breast Cancer Histopathology
School of Computer Science and Engineering（计算机科学与工程学院）；University of New South Wales（新南威尔士大学）；St. George and Sutherland Clinical School（圣乔治与萨瑟兰临床学院）；Department of Anatomical Pathology（解剖病理学部）；NSW Health Pathology（新南威尔士州卫生病理学）；Cancer Care Centre（癌症护理中心）
University of Geneva (UNIGE)（日内瓦大学）；University of Applied Sciences Western Switzerland (HES-SO)（瑞士西部应用科学大学）
Explanations for deep neural network predictions in terms of domain-related concepts can be valuable in medical applications, where justifications are important for confidence in the decision-making. In this work, we propose a methodology to exploit continuous concept measures as Regression Concept Vectors (RCVs) in the activation space of a layer. The directional derivative of the decision function along the RCVs represents the network sensitivity to increasing values of a given concept measure. When applied to breast cancer grading, nuclei texture emerges as a relevant concept in the detecti…
Leveraging Medical Foundation Model Features in Graph Neural Network-Based Retrieval of Breast Histopathology Images
Artificial Intelligence Department, Faculty of Computer Engineering, University of Isfahan, Isfahan, Iran；Department of Computer Engineering Techniques, Mazaya University College, Nasiriyah, Iraq；Department of Medicine, Danube Private University, Krems an der Donau, Austria；Austrian Center for Medical Innovation；Research Center for Medical Image Analysis；Artificial Intelligence, Department of Medicine, Danube Private University, Krems an der Donau, Austria
Automating tumor-infiltrating lymphocyte assessment in breast cancer histopathology images using QuPath: a transparent and accessible machine learning pipeline
UiT The Arctic University of Norway（挪威北极圈大学）；University Hospital of North Norway（挪威北部大学医院）
Multi-instance multi-label (MIML) learning is a challenging problem in many aspects. Such learning approaches might be useful for many medical diagnosis applications including breast cancer detection and classification. In this study subset of digiPATH dataset (whole slide digital breast cancer histopathology images) are used for training and evaluation of six state-of-the-art MIML methods. At the end, performance comparison of these approaches are given by means of effective evaluation metrics. It is shown that MIML-kNN achieve the best performance that is %65.3 average precision, where most …
Multi-modal Knowledge Decomposition based Online Distillation for Biomarker Prediction in Breast Cancer Histopathology
School of Biomedical Engineering, Faulty of Medicine, Dalian University of Technology, Dalian, China（生物医学工程学院）；Faculty of Information Technology, University of Jyvaskyla, Jyvaskyla, Finland（信息科技学院）；School of Software Technology, Dalian University of Technology, Dalian, China（软件技术学院）；Department of Radiology, Guangdong Provincial People’s Hospital, Southern Medical University, Guangzhou, China（放射科）
Eindhoven University of Technology（埃因霍温理工大学）；University Medical Center Utrecht（乌得勒支大学医学中心）；University Utrecht（乌得勒支大学）；Harvard Medical School（哈佛医学院）；Beth Israel Deaconess Medical Center（贝斯以色列女执事医疗中心）
Ductal carcinoma in situ (DCIS) is a non-invasive breast cancer that can progress into invasive ductal carcinoma (IDC). Studies suggest DCIS is often overtreated since a considerable part of DCIS lesions may never progress into IDC. Lower grade lesions have a lower progression speed and risk, possibly allowing treatment de-escalation. However, studies show significant inter-observer variation in DCIS grading. Automated image analysis may provide an objective solution to address high subjectivity of DCIS grading by pathologists. In this study, we developed a deep learning-based DCIS grading sys…
In this paper, we develop a complete pipeline for stain normalization, segmentation, and classification of nuclei in hematoxylin and eosin (H&E) stained breast cancer histopathology images. In the first step, we use a CNN-based stain transfer technique to normalize the staining characteristics of (H&E) images. We then train a neural network to segment images of nuclei from the H&E images. Finally, we train an Information Maximizing Generative Adversarial Network (InfoGAN) to learn visual representations of different types of nuclei and classify them in an entirely unsupervised manner. The resu…
Cancer is the uncontrollable cell division of abnormal cells inside the human body, which can spread to other body organs. It is one of the non-communicable diseases (NCDs) and NCDs accounts for 71% of total deaths worldwide whereas lung cancer is the second most diagnosed cancer after female breast cancer. Cancer survival rate of lung cancer is only 19%. There are various methods for the diagnosis of lung cancer, such as X-ray, CT scan, PET-CT scan, bronchoscopy and biopsy. However, to know the subtype of lung cancer based on the tissue type H and E staining is widely used, where the staining…
It is commonly recognized that color variations caused by differences in stains is a critical issue for histopathology image analysis. Existing methods adopt color matching, stain separation, stain transfer or the combination of them to alleviate the stain variation problem. In this paper, we propose a novel Stain-Adaptive Self-Supervised Learning(SASSL) method for histopathology image analysis. Our SASSL integrates a domain-adversarial training module into the SSL framework to learn distinctive features that are robust to both various transformations and stain variations. The proposed SASSL i…
The size of nuclei in histological preparations from excised breast tumors is predictive of patient outcome (large nuclei indicate poor outcome). Pathologists take into account nuclear size when performing breast cancer grading. In addition, the mean nuclear area (MNA) has been shown to have independent prognostic value. The straightforward approach to measuring nuclear size is by performing nuclei segmentation. We hypothesize that given an image of a tumor region with known nuclei locations, the area of the individual nuclei and region statistics such as the MNA can be reliably computed direc…
Vietnam Aviation Academy（越南航空学院）；Ho Chi Minh City Open University（胡志明市开放大学）；University of Information Technology, VNU-HCM（胡志明市国家大学信息技术大学）
According to some medical imaging techniques, breast histopathology images called Hematoxylin and Eosin are considered as the gold standard for cancer diagnoses. Based on the idea of dividing the pathologic image (WSI) into multiple patches, we used the window [512,512] sliding from left to right and sliding from top to bottom, each sliding step overlapping by 50% to augmented data on a dataset of 400 images which were gathered from the ICIAR 2018 Grand Challenge. Then use the EffficientNet model to classify and identify the histopathological images of breast cancer into 4 types: Normal, Benig…
Performance of externally validated machine learning models based on histopathology images for the diagnosis, classification, prognosis, or treatment outcome prediction in female breast cancer: A systematic review
McMaster University（麦克马斯特大学）；Mayo Clinic（梅奥诊所）；William Osler Health System（威廉·奥斯勒医疗系统）
Indian Institute of Technology Bombay（印度理工学院孟买分校）；University of Illinois（伊利诺伊大学）
Spatial arrangement of cells of various types, such as tumor infiltrating lymphocytes and the advancing edge of a tumor, are important features for detecting and characterizing cancers. However, convolutional neural networks (CNNs) do not explicitly extract intricate features of the spatial arrangements of the cells from histopathology images. In this work, we propose to classify cancers using graph convolutional networks (GCNs) by modeling a tissue section as a multi-attributed spatial graph of its constituent cells. Cells are detected using their nuclei in H&E stained tissue image, and each …
Histopathology image embedding is an active research area in computer vision. Most of the embedding models exclusively concentrate on a specific magnification level. However, a useful task in histopathology embedding is to train an embedding space regardless of the magnification level. Two main approaches for tackling this goal are domain adaptation and domain generalization, where the target magnification levels may or may not be introduced to the model in training, respectively. Although magnification adaptation is a well-studied topic in the literature, this paper, to the best of our knowle…
Reduced Deep Convolutional Activation Features (R-DeCAF) in Histopathology Images to Improve the Classification Performance for Breast Cancer Diagnosis
Institute of Medical Science and Technology, Shahid Beheshti University（沙希德·贝赫什提大学医学科学与技术研究所）
University of Memphis（孟菲斯大学）；Microsoft Research（微软研究院）；AutoML Image Lab（AutoML图像实验室）
Breast Cancer is the most prevalent cancer in the world. The World Health Organization reports that the disease still affects a significant portion of the developing world citing increased mortality rates in the majority of low to middle income countries. The most popular protocol pathologists use for diagnosing breast cancer is the Nottingham grading system which grades the proliferation of tumors based on 3 major criteria, the most important of them being mitotic cell count. The way in which pathologists evaluate mitotic cell count is to subjectively and qualitatively analyze cells present i…
Umm Al-Qura University（乌姆·库拉大学）；King Abdulaziz University（阿卜杜勒阿齐兹国王大学）；University of Tabuk（塔布克大学）；King Faisal Specialist Hospital and Research Center（费萨尔国王专科医院和研究中心）；University of Nevada（内华达大学）
Breast cancer is the most common cancer in the world and the second most common type of cancer that causes death in women. The timely and accurate diagnosis of breast cancer using histopathological images is crucial for patient care and treatment. Pathologists can make more accurate diagnoses with the help of a novel approach based on image processing. This approach is an ensemble model of two types of pre-trained vision transformer models, namely, Vision Transformer and Data-Efficient Image Transformer. The proposed ensemble model classifies breast cancer histopathology images into eight clas…
Addressing Uncertainty in Imbalanced Histopathology Image Classification of HER2 Breast Cancer: An interpretable Ensemble Approach with Threshold Filtered Single Instance Evaluation (SIE)
American International University-Bangladesh（孟加拉美国国际大学）；Bangladesh University of Business and Technology（孟加拉商业技术大学）；King Saud University（沙特国王大学）；Southern Illinois University（南伊利诺伊大学）
North Shore Country Day（北岸乡村日校）；Northwestern University（西北大学）
Data labeling is often the most challenging task when developing computational pathology models. Pathologist participation is necessary to generate accurate labels, and the limitations on pathologist time and demand for large, labeled datasets has led to research in areas including weakly supervised learning using patient-level labels, machine assisted annotation and active learning. In this paper we explore self-supervised learning to reduce labeling burdens in computational pathology. We explore this in the context of classification of breast cancer tissue using the Barlow Twins approach, an…
