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arXiv 2312.03396physics.optics
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高速脉冲受激布里渊显微镜

High-speed impulsive stimulated Brillouin microscopy

Jiarui Li, Taoran Le, Hongyuan Zhang, Haoyun Wei, Yan Li

AI总结:

本文通过SNR建模与相位、参考功率、采集时间多参数优化,并结合Matrix Pencil数据处理,构建高速ISBS显微镜,实现亚毫秒单像素成像和毫米级透明样品的无标记三维机械识别。

摘要:

布里渊显微镜通过散射光的频移来绘制弹性模量图,目前已发展到更快的成像速度,可用于研究快速的生物力学变化。脉冲受激布里渊散射(ISBS)光谱技术有望借助脉冲激发的共振放大相互作用和时域连续检测来加快测量速度。然而,由于信噪比(SNR)受限,以及为保持高光谱精度而需要进行过多平均,该技术尚未取得重大进展。此外,有限的空间分辨率也阻碍了其在机械成像中的应用。在这里,通过仔细研究SNR模型,我们通过包括相位、参考功率和采集时间在内的多参数优化,设计了一台高速ISBS显微镜。在此基础上,并进一步借助Matrix Pencil方法进行数据处理,我们针对浸没在甲醇中的毫米级聚二甲基硅氧烷图案,在多种对比机制下绘制了三维机械图像,从而能够在无任何接触或标记的情况下识别这两种透明材料。实验结果表明,该系统能够在单像素亚毫秒积分时间下保持高光谱精度和分辨率。与现有最先进系统相比,该方法在速度上提升了两个数量级,空间分辨率提高了十倍,使ISBS显微镜能够灵敏地研究时间和空间上的快速机械变化。

英文摘要:

Brillouin microscopy, which maps elastic modulus from the frequency shift of scattered light, has evolved to a faster speed for the investigation of rapid biomechanical changes. Impulsive stimulated Brillouin scattering (ISBS) spectroscopy has the potential to speed up measurement through the resonant amplification interaction from pulsed excitation and time-domain continuous detection. However, significant progress has not been achieved due to the limitation in signal-to-noise ratio (SNR) and the corresponding need for excessive averaging to maintain high spectral precision. Moreover, the limited spatial resolution also hinders its application in mechanical imaging. Here, by scrutinizing the SNR model, we design a high-speed ISBS microscope through multi-parameter optimization including phase, reference power, and acquisition time. Leveraging this, with the further assistance of the Matrix Pencil method for data processing, three-dimensional mechanical images are mapped under multiple contrast mechanisms for a millimeter-scale polydimethylsiloxane pattern immersed in methanol, enabling the identification of these two transparent materials without any contact or labeling. Our experimental results demonstrate the capability to maintain high spectral precision and resolution at a sub-millisecond integration time for one pixel. With a two-order improvement in the speed and a tenfold improvement in the spatial resolution over the state-of-the-art systems, this method makes it possible for ISBS microscopes to sensitively investigate rapid mechanical changes in time and space.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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